Development of genus/species -: Specific PCR analysis for identification of Carnobacterium strains

被引：90

作者：

Scarpellini, M

Mora, D

Colombo, S

Franzetti, L

机构：

[1] Univ Milan, Dipartimento Sci & Tecnol Alimentari & Microbiol, Sezione Microbiol Ind, I-20133 Milan, Italy

[2] Univ Milan, Dipartimento Sci & Tecnol Alimentari & Microbiol, Sezione Microbiol Agraria, I-20133 Milan, Italy

来源：

CURRENT MICROBIOLOGY | 2002年 / 45卷 / 01期

关键词：

D O I：

10.1007/s00284-001-0043-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Heterofermentative lactic acid bacteria belonging to the genus Carnobacterium are currently divided into seven different species, C. piscicola. C. mobile, C. gallinarum. C. inhibens, C. divergens, C. funditum, and C. alterfunditum. 16S rDNA-targeted PCR assay Was carried Out for the identification of the genus Carnobacterium In addition. type strains of all Carnobacterium species were analyzed by 16S-23S rDNA intergenic spacer analysis in comparison with type strains of phylogenetically related lactic acid bacteria. These methods enabled the identification and the discrimination among Carnobacterium species and the other phylogenetically related lactic acid bacteria. Likewise, analogous results were obtained by restriction analysis of amplified 16S rDNA performed with HaeIII and HinII as restriction enzymes.

引用

页码：24 / 29

页数：6

共 22 条

[1] USE OF THE POLYMERASE CHAIN-REACTION AND OLIGONUCLEOTIDE PROBES FOR THE RAPID DETECTION AND IDENTIFICATION OF CARNOBACTERIUM SPECIES FROM MEAT [J].

BROOKS, JL ;

MOORE, AS ;

PATCHETT, RA ;

COLLINS, MD ;

KROLL, RG .

JOURNAL OF APPLIED BACTERIOLOGY, 1992, 72 (04) :294-301

[2] CLASSIFICATION OF LACTOBACILLUS-DIVERGENS, LACTOBACILLUS-PISCICOLA, AND SOME CATALASE-NEGATIVE, ASPOROGENOUS, ROD-SHAPED BACTERIA FROM POULTRY IN A NEW GENUS, CARNOBACTERIUM [J].

COLLINS, MD ;

FARROW, JAE ;

PHILLIPS, BA ;

FERUSU, S ;

JONES, D .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1987, 37 (04) :310-316

[3]

COLLINS MD, 1991, FEMS MICROBIOL LETT, V77, P5, DOI [10.1016/S0378-1097(99)00289-X, 10.1111/j.1574-6968.1991.tb04313.x]

[4] 16S-23S rRNA internal transcribed spacers as molecular markers for the species of the 16S rRNA group I of the genus Bacillus [J].

Daffonchio, D ;

Borin, S ;

Consolandi, A ;

Mora, D ;

Manachini, PL ;

Sorlini, C .

FEMS MICROBIOLOGY LETTERS, 1998, 163 (02) :229-236

[5] PCR fingerprinting of whole genomes:: the spacers between the 16S and 23S rRNA genes and of intergenic tRNA gene regions reveal a different intraspecific genomic variability of Bacillus cereus and Bacillus licheniformis [J].

Daffonchio, D ;

Borin, S ;

Frova, G ;

Manachini, PL ;

Sorlini, C .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1998, 48 :107-116

[6] Homoduplex and heteroduplex polymorphisms of the amplified ribosomal 16S-23S internal transcribed spacers describe genetic relationships in the "Bacillus cereus group" [J].

Daffonchio, D ;

Cherif, A ;

Borin, S .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (12) :5460-5468

[7] DIFFERENTIATION OF CARNOBACTERIUM-DIVERGENS AND CARNOBACTERIUM-PISCICOLA BY NUMERICAL-ANALYSIS OF TOTAL SOLUBLE CELL PROTEIN-PATTERNS AND DNA-DNA HYBRIDIZATIONS [J].

DICKS, LMT ;

JANSSEN, B ;

DELLAGLIO, F .

CURRENT MICROBIOLOGY, 1995, 31 (02) :77-79

[8] PSYCHOTROPHIC, LACTIC ACID-PRODUCING BACTERIA FROM ANOXIC WATERS IN ACE LAKE, ANTARCTICA - CARNOBACTERIUM-FUNDITUM SP-NOV AND CARNOBACTERIUM-ALTERFUNDITUM SP-NOV [J].

FRANZMANN, PD ;

HOPFL, P ;

WEISS, N ;

TINDALL, BJ .

ARCHIVES OF MICROBIOLOGY, 1991, 156 (04) :255-262

[9]

HAMNES WP, 1991, POKARYOTES, V2, P1535

[10] Characteristics and genetic determinants of bacteriocin activities produced by Carnobacterium piscicola CP5 isolated from cheese [J].

Herbin, S ;

Mathieu, F ;

Brule, F ;

Branlant, C ;

Lefebvre, G ;

Lebrihi, A .

CURRENT MICROBIOLOGY, 1997, 35 (06) :319-326

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