Genetic analysis of turbot pathogenic Streptococcus parauberis strains by ribotyping and random amplified polymorphic DNA

被引：35

作者：

Romalde, JL ^{[1
]}

Magariños, B

Villar, C

Barja, JL

Toranzo, AE

机构：

[1] Univ Santiago Compostela, Fac Biol, Dept Microbiol & Parasitol, Santiago De Compostela 15706, Spain

[2] Univ Santiago Compostela, Inst Acuicultura, Santiago De Compostela 15706, Spain

来源：

FEMS MICROBIOLOGY LETTERS | 1999年 / 179卷 / 02期

关键词：

Streptococcus parauberis; ribotyping; random amplified polymorphic DNA analysis intraspecies classification; epidemiology;

D O I：

10.1111/j.1574-6968.1999.tb08741.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Ribotyping and RAPD profiling of a collection of 18 Streptococcus parauberis strains isolated from diseased turbot in Galicia (NW Spain) was performed in order to analyze the possible genetic variability within this bacterial fish pathogen. In addition, the value of this technique for intraspecific classification and epidemiological studies was evaluated. Ribopatterns of DNA digested with three endonucleases and hybridized with a cDNA probe complementary to highly conserved sequences in the 16S and 23S rRNA genes showed a great homogeneity among the turbot isolates. Compared with ribotyping, RAPD appeared to be a reliable and fast technique for discriminating between isolates of S. parauberis on the basis of their farm of isolation and, therefore, represents a powerful tool for epidemiological studies of this fish pathogen. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：297 / 304

页数：8

共 23 条

[1] Intraspecific differentiation of Vibrio vulnificus biotypes by amplified fragment length polymorphism and ribotyping [J].