Selenoprotein W is a glutathione-dependent antioxidant in vivo

被引：138

作者：

Jeong, DW

Kim, TS

Chung, YW

Lee, BJ

Kim, IY ^{[1
]}

机构：

[1] Korea Univ, Grad Sch Biotechnol, Lab Cellular & Mol Biochem, Seoul 136701, South Korea

[2] Seoul Natl Univ, Sch Biol Sci, Genet Mol Lab, Seoul 151742, South Korea

来源：

FEBS LETTERS | 2002年 / 517卷 / 1-3期

关键词：

selenoprotein W; selenocysteine; glutathione; hydrogen peroxide; antioxidant;

D O I：

10.1016/S0014-5793(02)02628-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The function of selenoprotein W (Se-W) was investigated by cloning the corresponding cDNA from mouse brain and expressing it in CHO cells and H1299 human lung cancer cells. Overexpression of Se-W markedly reduced the sensitivity of both cell lines to H2O2 cytotoxicity. The intracellular peroxide concentration of the transfected cells was lower than that of the parental cells in the absence or presence of extracellular H2O2. The resistance to oxidative stress conferred by Se-W was dependent on glutathione. Expression of Se-W mutants in which selenocysteine-13 or cysteine-37 was replaced by serine did not confer resistance to 14202, implicating these residues in the antioxidant activity of Se-W in vivo. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：225 / 228

页数：4

共 23 条

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