Biological molecule-impregnated polyester: An in vivo angiogenesis study

被引:29
作者
Fournier, N [1 ]
Doillon, CJ [1 ]
机构
[1] UNIV LAVAL,ST FRANCOIS ASSISE HOSP,INST BIOMAT,DEPT SURG,QUEBEC CITY,PQ G1K 7P4,CANADA
关键词
angiogenesis; Dacron(R); fibrin; growth factors;
D O I
10.1016/0142-9612(96)87645-9
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Specific extracellular matrix molecules and growth factors (GFs) with angiogenic properties could be combined with biomaterials to enhance angiogenesis and subsequently tissue ingrowth through the wall of the porous structure. In this study, composite fibrin matrices containing hyaluronic acid (HA), fibronectin (FN) and/or fibroblast growth factor-1 (FGF-1), FGF-2 and an endothelial cell growth supplement (EGGS) were adsorbed onto Dacron(R) meshes which were then implanted subcutaneously in mice. The release from the implants and the tissue distribution of implanted GFs were determined in vivo using radiolabelled FGF-2. Angiogenesis was quantified by counting the number of capillaries present in each Dacron(R) histological serial section. Radiolabelled GF was rapidly released from matrices and was absent from them by day 28. A very low percentage of the implanted radiolabelled GFs was found in the kidneys and livers of the animals. The number of microvessels formed within fibrin-impregnated samples was increased in the presence of HA and EGGS at 14d and of FN and EGGS or FGF-2 at 28d. FGF-1 had no direct effect on angiogenesis in our model. These results indicate that. enhancement of vascularization within prosthesis mesh may be achieved by using fibrin as a support for angiogenic molecules such as HA, FN and FGFs. (C) 1996 Elsevier Science Limited
引用
收藏
页码:1659 / 1665
页数:7
相关论文
共 33 条
[1]   ENHANCED ANGIOGENESIS AND GROWTH OF COLLATERALS BY INVIVO ADMINISTRATION OF RECOMBINANT BASIC FIBROBLAST GROWTH-FACTOR IN A RABBIT MODEL OF ACUTE LOWER-LIMB ISCHEMIA - DOSE-RESPONSE EFFECT OF BASIC FIBROBLAST GROWTH-FACTOR [J].
BAFFOUR, R ;
BERMAN, J ;
GARB, JL ;
RHEE, SW ;
KAUFMAN, J ;
FRIEDMANN, P .
JOURNAL OF VASCULAR SURGERY, 1992, 16 (02) :181-191
[2]   HEPARIN-DERIVED OLIGOSACCHARIDES - AFFINITY FOR ACIDIC FIBROBLAST GROWTH-FACTOR AND EFFECT ON ITS GROWTH-PROMOTING ACTIVITY FOR HUMAN-ENDOTHELIAL CELLS [J].
BARZU, T ;
LORMEAU, JC ;
PETITOU, M ;
MICHELSON, S ;
CHOAY, J .
JOURNAL OF CELLULAR PHYSIOLOGY, 1989, 140 (03) :538-548
[3]   STRUCTURAL EVIDENCE THAT ENDOTHELIAL-CELL GROWTH-FACTOR-BETA IS THE PRECURSOR OF BOTH ENDOTHELIAL-CELL GROWTH FACTOR-ALPHA AND ACIDIC FIBROBLAST GROWTH-FACTOR [J].
BURGESS, WH ;
MEHLMAN, T ;
MARSHAK, DR ;
FRASER, BA ;
MACIAG, T .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (19) :7216-7220
[4]  
CUEVAS P, 1992, INT J ARTIF ORGANS, V15, P360
[5]  
DOILLON CJ, 1994, MAT SCI ENG C-BIOMIM, V2, P43, DOI 10.1016/0928-4931(94)90028-0
[6]  
DVORAK HF, 1987, LAB INVEST, V57, P673
[7]  
FOURNIER N, 1994, CELL MATER, V4, P399
[8]  
FOURNIER N, 1992, CELL BIOL INT REP, V26, P1251
[9]   HEPARIN PROTECTS BASIC AND ACIDIC FGF FROM INACTIVATION [J].
GOSPODAROWICZ, D ;
CHENG, J .
JOURNAL OF CELLULAR PHYSIOLOGY, 1986, 128 (03) :475-484
[10]  
GREISLER HP, 1992, SURGERY, V112, P244