Circulating microRNAs as stable blood-based markers for cancer detection

被引：6511

作者：

Mitchell, Patrick S. ^{[1
]}

Parkin, Rachael K. ^{[1
]}

Kroh, Evan M. ^{[1
]}

Fritz, Brian R. ^{[1
]}

Wyman, Stacia K. ^{[1
]}

Pogosova-Agadjanyan, Era L. ^{[2
]}

Peterson, Amelia ^{[1
]}

Noteboom, Jennifer ^{[5
]}

O'Briant, Kathy C. ^{[3
]}

Allen, April ^{[3
]}

Lin, Daniel W. ^{[3
,5
,6
]}

Urban, Nicole ^{[3
]}

Drescher, Charles W. ^{[3
]}

Knudsen, Beatrice S. ^{[3
]}

Stirewalt, Derek L. ^{[2
]}

Gentleman, Robert ^{[3
]}

Vessella, Robert L. ^{[5
,6
]}

Nelson, Peter S. ^{[2
]}

Martin, Daniel B. ^{[1
,4
]}

Tewari, Muneesh ^{[1
,2
]}

机构：

[1] Fred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA

[2] Fred Hutchinson Canc Res Ctr, Div Clin Res, Seattle, WA 98109 USA

[3] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Seattle, WA 98109 USA

[4] Inst Syst Biol, Seattle, WA 98103 USA

[5] Univ Washington, Dept Urol, Seattle, WA 98195 USA

[6] Puget Sound Hlth Care System, Dept Vet Affairs, Seattle, WA 98108 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2008年 / 105卷 / 30期

关键词：

biomarker; miR-141; plasma; serum; prostate cancer;

D O I：

10.1073/pnas.0804549105

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

improved approaches for the detection of common epithelial malignancies are urgently needed to reduce the worldwide morbidity and mortality caused by cancer. MicroRNAs (miRNAs) are small (approximate to 22 nt) regulatory RNAs that are frequently dysregulated in cancer and have shown promise as tissue-based markers for cancer classification and prognostication. We show here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity. miRNAs originating from human prostate cancer xenografts enter the circulation, are readily measured in plasma, and can robustly distinguish xenografted mice from controls. This concept extends to cancer in humans, where serum levels of miR-141 (a miRNA expressed in prostate cancer) can distinguish patients with prostate cancer from healthy controls. Our results establish the measurement of tumor-derived miRNAs in serum or plasma as an important approach for the blood-based detection of human cancer.

引用

页码：10513 / 10518

页数：6

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