Gene expression profiling and pathway analysis of human bronchial epithelial cells exposed to airborne particulate matter collected from Saudi Arabia

被引：40

作者：

Sun, Hong ^{[1
]}

Shamy, Magdy ^{[2
]}

Kluz, Thomas ^{[1
]}

Munoz, Alexandra B. ^{[1
]}

Zhong, Mianhua ^{[1
]}

Laulicht, Freda ^{[1
]}

Alghamdi, Mansour A. ^{[2
]}

Khoder, Mamdouh I. ^{[2
]}

Chen, Lung-Chi ^{[1
]}

Costa, Max ^{[1
]}

机构：

[1] NYU, Sch Med, Dept Environm Med, Tuxedo Pk, NY 10987 USA

[2] King Abdulaziz Univ, Fac Meteorol Environm & Arid Land Agr, Dept Environm Sci, Jeddah 21413, Saudi Arabia

来源：

TOXICOLOGY AND APPLIED PHARMACOLOGY | 2012年 / 265卷 / 02期

关键词：

Particulate matter; Gene expression; Human bronchial epithelial cells; Microarray; Ingenuity Pathway Analysis; ENDOPLASMIC-RETICULUM STRESS; AIR-POLLUTION; LUNG; SREBP; BINDING; URBAN; PARTICLES; AMBIENT; MICE; FINE;

D O I：

10.1016/j.taap.2012.10.008

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Epidemiological studies have established a positive correlation between human mortality and increased concentration of airborne particulate matters (PM). However, the mechanisms underlying PM related human diseases, as well as the molecules and pathways mediating the cellular response to PM, are not fully understood. This study aims to investigate the global gene expression changes in human cells exposed to PM10 and to identify genes and pathways that may contribute to PM related adverse health effects. Human bronchial epithelial cells were exposed to PM10 collected from Saudi Arabia for 1 or 4 days, and whole transcript expression was profiled using the GeneChip human gene 1.0 ST array. A total of 140 and 230 genes were identified that significantly changed more than 1.5 fold after PM10 exposure for 1 or 4 days, respectively. Ingenuity Pathway Analysis revealed that different exposure durations triggered distinct pathways. Genes involved in NRF2-mediated response to oxidative stress were up-regulated after 1 day exposure. In contrast, cells exposed for 4 days exhibited significant changes in genes related to cholesterol and lipid synthesis pathways. These observed changes in cellular oxidative stress and lipid synthesis might contribute to PM related respiratory and cardiovascular disease. (c) 2012 Elsevier Inc. All rights reserved.

引用

页码：147 / 157

页数：11

共 52 条

[1] Cytotoxic and proinflammatory effects of ambient and source-related particulate matter (PM) in relation to the production of reactive oxygen species (ROS) and cytokine adsorption by particles [J].

Akhtar, Umme S. ;

McWhinney, Robert D. ;

Rastogi, Neeraj ;

Abbatt, Jonathan P. D. ;

Evans, Greg J. ;

Scott, Jeremy A. .

INHALATION TOXICOLOGY, 2010, 22 :37-47

[2]

Akhtar US, 2010, ENVIRON SCI ENG, P427, DOI 10.1007/978-3-642-12278-1_22

[3] Particulate matter and atherosclerosis: role of particle size, composition and oxidative stress [J].

Araujo, Jesus A. ;

Nel, Andre E. .

PARTICLE AND FIBRE TOXICOLOGY, 2009, 6

[4] Seasonal variations in air pollution particle-induced inflammatory mediator release and oxidative stress [J].

Becker, S ;

Dailey, LA ;

Soukup, JM ;

Grambow, SC ;

Devlin, RB ;

Huang, YCT .

ENVIRONMENTAL HEALTH PERSPECTIVES, 2005, 113 (08) :1032-1038

[5] Reassessment of the lethal London fog of 1952: Novel indicators of acute and chronic consequences of acute exposure to air pollution [J].

Bell, ML ;

Davis, DL .

ENVIRONMENTAL HEALTH PERSPECTIVES, 2001, 109 :389-394

[6] SREBP in signal transduction: cholesterol metabolism and beyond [J].

Bengoechea-Alonso, Maria T. ;

Ericsson, Johan .

CURRENT OPINION IN CELL BIOLOGY, 2007, 19 (02) :215-222

[7] Deletion of Scap in Alveolar Type II Cells Influences Lung Lipid Homeostasis and Identifies a Compensatory Role for Pulmonary Lipofibroblasts [J].

Besnard, Valerie ;

Wert, Susan E. ;

Stahlman, Mildred T. ;

Postle, Anthony D. ;

Xu, Yan ;

Ikegami, Machiko ;

Whitsett, Jeffrey A. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (06) :4018-4030

[8] Novel role for the liver X nuclear receptor in the suppression of lung inflammatory responses [J].

Birrell, Mark A. ;

Catley, Matthew C. ;

Hardaker, Elizabeth ;

Wong, Sissie ;

Willson, Timothy M. ;

McCluskie, Kerryn ;

Leonard, Thomas ;

Farrow, Stuart N. ;

Collins, Jon L. ;

Haj-Yahia, Saleem ;

Belvisi, Maria G. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (44) :31882-31890

[9] Particulate Matter Air Pollution and Cardiovascular Disease An Update to the Scientific Statement From the American Heart Association [J].

Brook, Robert D. ;

Rajagopalan, Sanjay ;

Pope, C. Arden, III ;

Brook, Jeffrey R. ;

Bhatnagar, Aruni ;

Diez-Roux, Ana V. ;

Holguin, Fernando ;

Hong, Yuling ;

Luepker, Russell V. ;

Mittleman, Murray A. ;

Peters, Annette ;

Siscovick, David ;

Smith, Sidney C., Jr. ;

Whitsel, Laurie ;

Kaufman, Joel D. .

CIRCULATION, 2010, 121 (21) :2331-2378

[10] Effects of Metals within Ambient Air Particulate Matter (PM) on Human Health [J].

Chen, Lung Chi ;

Lippmann, Morton .

INHALATION TOXICOLOGY, 2009, 21 (01) :1-31

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