Laser capture microdissection and mRNA characterization of mouse airway epithelium: methodological considerations

被引：20

作者：

Betsuyaku, T ^{[1
]}

Senior, RM

机构：

[1] Hokkaido Univ, Sch Med, Dept Med 1, Sapporo, Hokkaido, Japan

[2] Univ Washington, Sch Med, Dept Med, St Louis, MO USA

[3] Univ Washington, Sch Med, Dept Cell Biol & Physiol, St Louis, MO USA

来源：

MICRON | 2004年 / 35卷 / 04期

关键词：

clara cell; nissl stain; real-time polymerase chain reaction; microarray;

D O I：

10.1016/j.micron.2003.11.015

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

The use of laser capture microdissection (LCM) to obtain epithelial cells lining the distal airways for gene profiling is described. In the mouse, the distal airways are particularly attractive for LCM as there is very high percentage of a single cell type, Clara cells, lining these airways. It is shown that the RNA from distal airway epithelial cells harvested by LCM is well preserved and that with linear amplification sufficient cRNA for microarray analysis can be attained from small numbers of cells. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：229 / 234

页数：6

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