Evaluation of the Infectiousness to Mice of Soil Contaminated with Yersinia pestis-Infected Blood

被引:20
作者
Boegler, Karen A. [1 ]
Graham, Christine B. [1 ]
Montenieri, John A. [1 ]
MacMillan, Katherine [1 ]
Holmes, Jennifer L. [1 ]
Petersen, Jeannine M. [1 ]
Gage, Kenneth L. [1 ]
Eisen, Rebecca J. [1 ]
机构
[1] Ctr Dis Control & Prevent, Div Vector Borne Dis, Ft Collins, CO 80521 USA
关键词
Plague; Soil; Transmission; Yersinia pestis; PLAGUE; PERSISTENCE; FIELD;
D O I
10.1089/vbz.2012.1031
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Plague, an often-fatal zoonotic disease caused by Yersinia pestis, is characterized by epizootic and quiescent periods. How Y. pestis is maintained during inter-epizootic periods is poorly understood, but soil has been implicated as a potential reservoir. Although previous studies have suggested that Y. pestis is able to survive in soil for weeks or months, it is unclear whether or not it is infectious to susceptible hosts. Here we investigate the potential for Y. pestis to infect mice through close contact with contaminated soil under laboratory conditions. In an attempt to approximate the natural conditions under which animals would be exposed to Y. pestis-contaminated soil, mouse cages filled with soil from a plague-endemic region were held at temperature and humidity ranges observed in ground squirrel burrows. These laboratory "burrows" were contaminated with highly bacteremic blood (>10(8) cfu/mL) to simulate the introduction of infectious material from a dying animal during an epizootic. Outbred Swiss-Webster mice with scarified skin patches were held on contaminated soil for 10 days and monitored for signs of illness. Following exposure to contaminated soil, one animal of 104 became infected with Y. pestis. None of the remaining animals seroconverted following a 21-day holding period. Under our experimental conditions, which maximized the likelihood of contact between susceptible mice and contaminated soil, transmission efficiency from soil to mice was 0.96% (95% CI 0.17, 5.25%). This suggests that although transmission of Y. pestis from contaminated soils is possible, it is not likely a major transmission route under natural conditions.
引用
收藏
页码:948 / 952
页数:5
相关论文
共 28 条
  • [1] Yersinia pestis, the cause of plague, is a recently emerged clone of Yersinia pseudotuberculosis
    Achtman, M
    Zurth, K
    Morelli, C
    Torrea, G
    Guiyoule, A
    Carniel, E
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (24) : 14043 - 14048
  • [2] Long-term persistence of virulent Yersinia pestis in soil
    Ayyadurai, Saravanan
    Houhamdi, Linda
    Lepidi, Hubert
    Nappez, Claude
    Raoult, Didier
    Drancourt, Michel
    [J]. MICROBIOLOGY-SGM, 2008, 154 : 2865 - 2871
  • [3] BALTAZARD M., 1963, BULL SOC PATHOL EXOTIQUE, V56, P1230
  • [4] ENERGY METABOLISM AND EVAPORATIVE WATER-LOSS IN CALIFORNIA GROUND SQUIRREL - EFFECTS OF BURROW TEMPERATURE AND WATER VAPOR-PRESSURE
    BAUDINETTE, RV
    [J]. JOURNAL OF COMPARATIVE PHYSIOLOGY, 1972, 81 (01): : 57 - +
  • [5] Bazanova L P, 1997, Med Parazitol (Mosk), P37
  • [6] Bazanova L. P., 2007, Meditsinskaya Parazitologiya i Parazitarnye Bolezni, P34
  • [7] Experimental infection of ground squirrels (Citellus pygmaeus Pallas) with Yersinia pestis during hibernation
    Bizanov, Gazim
    Dobrokhotova, Nina D.
    [J]. JOURNAL OF INFECTION, 2007, 54 (02) : 198 - 203
  • [8] Centers for Disease Control and Prevention for PulseNet USA, 2006, ON DAY 24 28 H STAND
  • [9] Insights into the evolution of Yersinia pestis through whole-genome comparison with Yersinia pseudotuberculosis
    Chain, PSG
    Carniel, E
    Larimer, FW
    Lamerdin, J
    Stoutland, PO
    Regala, WM
    Georgescu, AM
    Vergez, LM
    Land, ML
    Motin, VL
    Brubaker, RR
    Fowler, J
    Hinnebusch, J
    Marceau, M
    Medigue, C
    Simonet, M
    Chenal-Francisque, V
    Souza, B
    Dacheux, D
    Elliott, JM
    Derbise, A
    Hauser, LJ
    Garcia, E
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (38) : 13826 - 13831
  • [10] Chu MC., 2000, Laboratory manual of plague diagnostics, P129