Effect of β-Cyclodextrin Complexation on Solubility and Enzymatic Conversion of Naringin

被引：71

作者：

Cui, Li ^{[1
,2
]}

Zhang, Zhen-Hai ^{[1
]}

Sun, E. ^{[1
]}

Jia, Xiao-Bin ^{[1
,2
]}

机构：

[1] Jiangsu Prov Acad Chinese Med, Key Lab New Drug Delivery Syst Chinese Meteria Me, Nanjing 210028, Jiangsu, Peoples R China

[2] Nanjing Univ Chinese Med, Nanjing 210046, Jiangsu, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2012年 / 13卷 / 11期

基金：

中国国家自然科学基金;

关键词：

beta-cyclodextrin; inclusion complex; enzymolysis; naringin; naringenin; INCLUSION COMPLEX; FLAVONOIDS; HYDROLYSIS; EXTRACTION;

D O I：

10.3390/ijms131114251

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

In the present paper, the effect of beta-cyclodextrin (beta-CD) inclusion complexation on the solubility and enzymatic hydrolysis of naringin was investigated. The inclusion complex of naringin/beta-CD at the molar ratio of 1: 1 was obtained by the dropping method and was characterized by differential scanning calorimetry. The solubility of naringin complexes in water at 37 +/- 0.1 degrees C was 15 times greater than that of free naringin. Snailase-involved hydrolysis conditions were tested for the bioconversion of naringin into naringenin using the univariate experimental design. Naringin can be transformed into naringenin by snailase-involved hydrolysis. The optimum conditions for enzymatic hydrolysis were determined as follows: pH 5.0, temperature 37 degrees C, ratio of snailase/substrate 0.8, substrate concentration 20 mg.mL(-1), and reaction time 12 h. Under the optimum conditions, the transforming rate of naringenin from naringin for inclusion complexes and free naringin was 98.7% and 56.2% respectively, suggesting that beta-CD complexation can improve the aqueous solubility and consequently the enzymatic hydrolysis rate of naringin.

引用

页码：14251 / 14261

页数：11

共 26 条

[1]

Naringenin attenuates cisplatin nephrotoxicity in rats [J].