Bacterial communities in iron mottles in the plow pan layer in a Japanese rice field: Estimation using PCR-DGGE and sequencing analyses

被引：15

作者：

Cahyani, Vita Ratri ^{[1
,2
]}

Murase, Jun ^{[2
]}

Ikeda, Akihiro ^{[3
]}

Taki, Katsutoshi ^{[3
]}

Asakawa, Susumu ^{[2
]}

Kimura, Makoto ^{[2
]}

机构：

[1] Sebelas Maret Univ, Fac Agr, Dept Soil Sci, Lab Soil Biol, Surakarta 57126, Jawa Tengah, Indonesia

[2] Nagoya Univ, Grad Sch Bioagr Sci, Nagoya, Aichi 4648601, Japan

[3] Aichi Ken Agr Res Ctr, Aichi 4801193, Japan

来源：

SOIL SCIENCE AND PLANT NUTRITION | 2008年 / 54卷 / 05期

基金：

日本学术振兴会;

关键词：

bacterial community; iron mottle; iron oxidation; iron reduction; rice field;

D O I：

10.1111/j.1747-0765.2008.00291.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Bacterial communities in iron (Fe) mottles in the plow pan layer in a Japanese rice field were estimated using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis targeting 16S rDNA genes. The DGGE band patterns indicate that distinct bacterial communities with lower diversity inhabit Fe mottles compared with the reference soil matrix. Many of the DGGE bands of the Fe mottles that were sequenced (12 of 29 DGGE bands) were closely related to bacteria involved with Fe oxidation and reduction: Siderooxidans ghiorsii, Azoarcus sp., Azovibrio sp., Dechloromonas sp., Acidimicrobium ferrooxidans, Geobacter psychrophilus, Clostridium sp., Desulfovibrio sp. and Desulfonatronum cooperativum. The results suggest that specific bacteria inhabit Fe mottles and may play a role in the Fe cycle.

引用

页码：711 / 717

页数：7

共 28 条

[1] TAXONOMIC POSITION OF AROMATIC-DEGRADING DENITRIFYING PSEUDOMONAD STRAINS K-172 AND KB-740 AND THEIR DESCRIPTION AS NEW MEMBERS OF THE GENERA THAUERA, AS THAUERA-AROMATICA SP-NOV, AND AZOARCUS, AS AZOARCUS-EVANSII SP-NOV, RESPECTIVELY, MEMBERS OF THE BETA-SUBCLASS OF THE PROTEOBACTERIA [J].