Application of liposomes incorporating doxorubicin with sialyl Lewis X to prevent stenosis after rat carotid artery injury

被引：22

作者：

Tsuruta, Wataro ^{[2
]}

Tsurushima, Hideo ^{[1
]}

Yamamoto, Tetsuya ^{[2
]}

Suzuki, Kensuke ^{[2
]}

Yamazaki, Noboru ^{[1
]}

Matsumura, Akira ^{[2
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Nanotechnol Res Inst, Tsukuba, Ibaraki 3058565, Japan

[2] Univ Tsukuba, Inst Clin Med, Dept Neurosurg, Tsukuba, Ibaraki 305, Japan

来源：

BIOMATERIALS | 2009年 / 30卷 / 01期

关键词：

Vascular stenosis; Drug delivery; Intimal hyperplasia; Liposome; Selectin; INTIMAL HYPERPLASIA; E-SELECTIN; RESTENOSIS; ANGIOPLASTY; INFLAMMATION; EFFICACY; CELLS;

D O I：

10.1016/j.biomaterials.2008.09.009

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Restenosis remains a serious complication that can occur after angioplasty. This study investigated the efficiency of an active targeting chemotherapy using liposomes, including doxorubicin, whose surface was decorated with sialyl Lewis X (SIX) (Dox-Lipo-SLX) to prevent stenosis after angioplasty. Its delivery was controlled via the affinity between SLX and E-selectin proteins, which are expressed on vessel walls with injury. In vitro experiments confirmed the accumulation of doxorubicin as a consequence of Dox-Lipo-SLX adhering to E-selectin-positive cells. Significant doxorubicin accumulation was observed on injured vessel walls in rats treated with Dox-Lipo-SLX. in contrast, there was little accumulation using free doxorubicin or a liposome containing doxorubicin (Dox-Lipo), but without SIX. Rats were assigned to one of four groups: Dox-Lipo-SLX, Dox-Lipo, free doxorubicin, or no treatment. Dox-Lipo-SLX, Dox-Lipo, and free doxorubicin, including a dose of 0.08 mg/kg doxorubicin, were intravenously administered three times in each group after angioplasty. The residual lumen area of rats in the group treated with Dox-Lipo-SLX was significantly larger than those in all other groups. These results demonstrate that an active targeting drug delivery system utilizing Dox-Lipo-SLX effectively prevents stenosis after angioplasty. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：118 / 125

页数：8

共 34 条

[1] Design criteria for the ideal drug-eluting Stent [J].

Ako, Junya ;

Bonneau, Heidi N. ;

Honda, Yasuhiro ;

Fitzgerald, Peter J. .

AMERICAN JOURNAL OF CARDIOLOGY, 2007, 100 (8B) :3M-9M

[2] A reappraisal of angioplasty and stenting for the treatment of vertebral origin stenosis [J].

Albuquerque, FC ;

Fiorella, D ;

Han, P ;

Spetzler, RF ;

McDougall, CG .

NEUROSURGERY, 2003, 53 (03) :607-614

[3] OPTIMIZATION AND UPSCALING OF DOXORUBICIN-CONTAINING LIPOSOMES FOR CLINICAL USE [J].

AMSELEM, S ;

GABIZON, A ;

BARENHOLZ, Y .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1990, 79 (12) :1045-1052

[4]

Bauters C, 1996, CARDIOVASC RES, V31, P835

[5] SELECTINS [J].

BEVILACQUA, MP ;

NELSON, RM .

JOURNAL OF CLINICAL INVESTIGATION, 1993, 91 (02) :379-387

[6]

BRUNEVAL P, 1986, ARCH PATHOL LAB MED, V110, P1186

[7]

CARLOS TM, 1994, BLOOD, V84, P2068

[8]

CARSON SN, 1981, SURG GYNECOL OBSTET, V153, P883

[9] Extracranial vertebral artery stent placement: in-hospital and follow-up results [J].

Chastain, HD ;

Campbell, MS ;

Iyer, S ;

Roubin, GS ;

Vitek, J ;

Mathur, A ;

Al-Mubarak, NA ;

Terry, JB ;

Yates, V ;

Kretzer, K ;

Alred, D ;

Gomez, CR .

JOURNAL OF NEUROSURGERY, 1999, 91 (04) :547-552

[10] Lectin-like proteins in model organisms: implications for evolution of carbohydrate-binding activity [J].

Dodd, RB ;

Drickamer, K .

GLYCOBIOLOGY, 2001, 11 (05) :71R-79R

← 1 2 3 4 →