Recombinant avian adenovirus CELO expressing the human interleukin-2: characterization in vitro, in ovo and in vivo

被引：14

作者：

Cherenova, LV

Logunov, DY

Shashkova, EV

Shmarov, MM

Verkhovskaya, L

Neugodova, GL

Kazansky, DB

Doronin, KK

Naroditsky, BS

机构：

[1] NF Gamalei Inst Epidemiol & Microbiol, Moscow, Russia

[2] Inst Agr Biotechnol, Moscow, Russia

[3] Russian NN Blokhin Canc Res Ctr, Inst Carcinogenesis, Moscow, Russia

来源：

VIRUS RESEARCH | 2004年 / 100卷 / 02期

基金：

俄罗斯基础研究基金会;

关键词：

avian adenovirus CELO; human interleukin-2 (IL-2); secreted placental alkaline phosphatase (SEAP); green fluorescent protein (GFP); recombinant adenovirus; B16; melanoma;

D O I：

10.1016/j.virusres.2003.11.018

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In our study, a recombinant adenovirus based on the avian adenovirus CELO genome, has been constructed that contains the human interleukin-2 gene. We have shown the production of biologically active recombinant interleukin-2 in vitro (LMH and 293 cells) and in ovo (chicken embryos) infected with recombinant vir-us CELO-IL2. An increase in the median survival time of C57BL/6 mice carrying B16 melanoma tumors has been demonstrated after multiple intra-tumors injections of the recombinant adenovirus CELO-IL2. (C) 2003 Elsevier B.V. All rights reserved.

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收藏

页码：257 / 261

页数：5

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