MUC17, a novel membrane-tethered mucin

被引:174
作者
Gum, JR
Crawley, SC
Hicks, JW
Szymkowski, DE
Kim, YS
机构
[1] Dept Vet Affairs Med Ctr, Gastrointestinal Res Lab, San Francisco, CA 94121 USA
[2] Univ Calif San Francisco, Dept Anat, San Francisco, CA 94143 USA
[3] Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
[4] Univ Calif San Francisco, Dept Pathol, San Francisco, CA 94143 USA
[5] Roche Biosci, Palo Alto, CA USA
关键词
EGF-like domain; SEA module domain; O-glycosylation; signal transduction; gene cluster; radiation hybrid mapping;
D O I
10.1006/bbrc.2002.6475
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Membrane mucins have several functions in epithelial cells including cytoprotection, extravasation during metastases, maintenance of luminal structure, and signal transduction. In this paper we describe a large membrane mucin expressed in the normal intestine. This novel mucin, designated MUC17, contains an extended, repetitive extracellular glycosylation domain and a carboxyl terminus with two EGF-like domains, a SEA module domain, a transmembrane domain, and a cytoplasmic domain with potential serine and tyrosine phosphorylation sites. RNA blot analysis and in situ hybridization indicates that MUC17 is expressed in select pancreatic and colon cancer cell lines and in intestinal absorptive cells. Radiation hybrid mapping localized MUC17 to chromosome 7q22 where it resides in close proximity with three other membrane mucin genes, MUC3A, MUC3B, and MUC12. Thus, these membrane mucins reside together in a gene cluster, but are expressed in different tissues and are likely to have different functions as well.
引用
收藏
页码:466 / 475
页数:10
相关论文
共 34 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]   Sequence and structure-based prediction of eukaryotic protein phosphorylation sites [J].
Blom, N ;
Gammeltoft, S ;
Brunak, S .
JOURNAL OF MOLECULAR BIOLOGY, 1999, 294 (05) :1351-1362
[3]   THE SEA MODULE - A NEW EXTRACELLULAR DOMAIN ASSOCIATED WITH O-GLYCOSYLATION [J].
BORK, P ;
PATTHY, L .
PROTEIN SCIENCE, 1995, 4 (07) :1421-1425
[4]   An intramembrane modulator of the ErbB2 receptor tyrosine kinase that potentiates neuregulin signaling [J].
Carraway, KL ;
Rossi, EA ;
Komatsu, M ;
Price-Schiavi, SA ;
Huang, DM ;
Guy, PM ;
Carvajal, ME ;
Fregien, N ;
Carraway, CAC ;
Carraway, KL .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (09) :5263-5266
[5]   Genomic organization and structure of the 3′ region of human MUC3:: Alternative splicing predicts membrane-bound and soluble forms of the mucin [J].
Crawley, SC ;
Gum, JR ;
Hicks, JW ;
Pratt, WS ;
Aubert, JP ;
Swallow, DM ;
Kim, YS .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 263 (03) :728-736
[6]   Human mucin genes MUC2, MUC3, MUC4, MUC5AC, MUC5B, and MUC6 express stable and extremely large mRNAs and exhibit a variable length polymorphism -: An improved method to analyze large mRNAs [J].
Debailleul, V ;
Laine, A ;
Huet, G ;
Mathon, P ;
d'Hooghe, MC ;
Aubert, JP ;
Porchet, N .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (02) :881-890
[7]   CLONING OF PARTIAL CDNA-ENCODING DIFFERENTIATION AND TUMOR-ASSOCIATED MUCIN GLYCOPROTEINS EXPRESSED BY HUMAN MAMMARY EPITHELIUM [J].
GENDLER, SJ ;
BURCHELL, JM ;
DUHIG, T ;
LAMPORT, D ;
WHITE, R ;
PARKER, M ;
TAYLORPAPADIMITRIOU, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (17) :6060-6064
[8]   MOLECULAR-CLONING OF CDNAS DERIVED FROM A NOVEL HUMAN INTESTINAL MUCIN GENE [J].
GUM, JR ;
HICKS, JW ;
SWALLOW, DM ;
LAGACE, RL ;
BYRD, JC ;
LAMPORT, DTA ;
SIDDIKI, B ;
KIM, YS .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 171 (01) :407-415
[9]   MUC3 human intestinal mucin - Analysis of gene structure, the carboxyl terminus, and a novel upstream repetitive region [J].
Gum, JR ;
Ho, JJL ;
Pratt, WS ;
Hicks, JW ;
Hill, AS ;
Vinall, LE ;
Roberton, AM ;
Swallow, DM ;
Kim, YS .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (42) :26678-26686
[10]   A radiation hybrid map of the human genome [J].
Gyapay, G ;
Schmitt, K ;
Fizames, C ;
Jones, H ;
VegaCzarny, N ;
Spillett, D ;
Muselet, D ;
PrudHomme, JF ;
Dib, C ;
Auffray, C ;
Morissette, J ;
Weissenbach, J ;
Goodfellow, PN .
HUMAN MOLECULAR GENETICS, 1996, 5 (03) :339-346