Cloning and characterization of a nonhemolytic phospholipase C gene from Burkholderia pseudomallei

被引：14

作者：

Korbsrisate, S

Suwanasai, N

Leelaporn, A

Ezaki, T

Kawamura, Y

Sarasombath, S

机构：

[1] Mahidol Univ, Siriraj Hosp, Fac Med, Dept Immunol, Bangkok 10700, Thailand

[2] Siriraj Hosp, Fac Med, Dept Microbiol, Bangkok 10700, Thailand

[3] Gifu Univ, Sch Med, Dept Microbiol, Gifu 500, Japan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 11期

关键词：

D O I：

10.1128/JCM.37.11.3742-3745.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We cloned and characterized a phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) gene from Burkholderia pseudomallei, DNA sequence analysis of the gene indicated an open reading frame coding for 700 amino acids with a 34-amino-acid signal peptide. When cleaved, this yields a secreted 73-kDa mature protein. The deduced amino acid sequence exhibited 48% similarity to that of a nonhemolytic PLC from Pseudomonas aeruginosa, The expressed PC-PLC was heat stable, nonhemolytic for sheep erythrocytes, and active between pH 2 and 8. Western blot analysis with sera from melioidosis patients indicated that they produced immunoglobulin M antibodies against this PC-PLC protein.

引用

页码：3742 / 3745

页数：4

共 25 条

[1] PRODUCTION OF HEMOLYSIN AND OTHER EXTRACELLULAR ENZYMES BY CLINICAL ISOLATES OF PSEUDOMONAS-PSEUDOMALLEI [J].