A truncated splice variant of human BARD1 that lacks the RING finger and ankyrin repeats

被引：23

作者：

Tsuzuki, M

Wu, WW

Nishikawa, H

Hayami, R

Oyake, D

Yabuki, Y

Fukuda, M

Ohta, T

机构：

[1] St Marianna Univ, Sch Med, Dept Surg, Div Breast & Endocrine Surg,Miyamae Ku, Kawasaki, Kanagawa 2168511, Japan

[2] St Marianna Univ, Sch Med, Dept Otorhinolaryngol, Kawasaki, Kanagawa 2168511, Japan

来源：

CANCER LETTERS | 2006年 / 233卷 / 01期

基金：

日本学术振兴会;

关键词：

BARD1; BRCA1; splicing variant; RING finger; ubiquitin;

D O I：

10.1016/j.canlet.2005.03.012

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

BARD1 is a crucial partner of the breast and ovarian tumor suppressor BRCA1 required for ubiquitin ligase activity and for reciprocal stabilization in cells. We report here an alternatively spliced human BARD1 mRNA variant (BARD1 Delta RIN) isolated from a HeLa cell cDNA library. It is characterized by deletion of exons 2-6 that encode most of the RING finger domain and the entire span of ankyrin repeats. Delta RIN transcript was detected in all breast cancer-cell lines studied although its protein expression level was low. Delta RIN does not interact with BRCA1, whereas it interacts with and colocalizes with CstF-50 to cytoplasmic dots. Hence, a deletion variant of BARD1 occurs in cells and may play a distinct role with CstF-50. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

引用

页码：108 / 116

页数：9

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