High-throughput single nucleotide polymorphism genotyping by fluorescent 5′ exonuclease assay

被引：74

作者：

Morin, PA ^{[1
]}

Saiz, R ^{[1
]}

Monjazeb, A ^{[1
]}

机构：

[1] Axys Pharmaceut Inc, La Jolla, CA 92037 USA

来源：

BIOTECHNIQUES | 1999年 / 27卷 / 03期

关键词：

D O I：

10.2144/99273rr02

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Single nucleotide polymorphisms (SNPs) are the class of DNA variants that are most common in the genome. Technologies are needed that allow relatively high-throughput, high-quality genotyping of SNPs, with rapid assay development for individual SNPs of interest. We have implemented an accurate and high-throughput SNP genotyping system using a commercially available fluorescent 5' exonuclease assay. Optimization of the assay system for low-volume reactions and low-probe concentrations has reduced assay costs by 75%. Using a simple assay optimization process, we successfully developed genotyping assays for 92% of assays attempted (309 out of 335) and have generated over 200 000 genotypes.

引用

页码：538 / +

页数：9

共 20 条

[1] Variations on a theme: Cataloging human DNA sequence variation [J].