Exploration of single molecule events in a haloperoxidase and its biomimic:: Localization of halogenation activity

被引：38

作者：

Martinez, Virginia Martinez ^{[1
]}

De Cremer, Gert ^{[2
]}

Roeffaers, Maarten B. J. ^{[1
,2
]}

Sliwa, Michel ^{[1
]}

Baruah, Mukulesh ^{[1
]}

De Vos, Dirk E. ^{[2
]}

Hofkens, Johan ^{[1
]}

Sels, Bert F. ^{[2
]}

机构：

[1] Katholieke Univ Leuven, Dept Chem, B-3001 Louvain, Belgium

[2] Katholieke Univ Leuven, Dept Microbial & Mol Syst, B-3001 Louvain, Belgium

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 40期

关键词：

D O I：

10.1021/ja804606m

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In situ observation of single oxidation/halogenation events by catalytically generated hypobromite species using single molecule fluorescence microscopy allows montioring of the diffusion behavior of these halonium species from the catalyst into the bulk solution. The fluororgenic probe specifically reacts with hypohalites, yielding fluorescein that can be detected with single molecule sensitivity. It was found for two investigated catalysts (Curvularia verruculosa enzymes and tungstate-exchanged LDH crystals) that in steady-state conditions hypobromite is able to diffuse over 800 nm in the bulk solution before it oxidizes organic substrates.

引用

页码：13192 / 13193

页数：2

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