Heritability of targeted gene modifications induced by plant-optimized CRISPR systems

被引：42

作者：

Mao, Yanfei ^{[1
]}

Botella, Jose Ramon ^{[2
]}

Zhu, Jian-Kang ^{[1
,3
]}

机构：

[1] Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 200032, Peoples R China

[2] Univ Queensland, Sch Agr & Food Sci, Brisbane, Qld 4072, Australia

[3] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA

来源：

CELLULAR AND MOLECULAR LIFE SCIENCES | 2017年 / 74卷 / 06期

关键词：

CRISPR-Cas; sgRNA; Gene editing; Mutagenesis; Inheritance; DOUBLE-STRAND BREAKS; INTRACHROMOSOMAL HOMOLOGOUS RECOMBINATION; FINGER DNA-RECOGNITION; TOBACCO-RATTLE-VIRUS; GUIDE RNA; IN-VIVO; ARABIDOPSIS-THALIANA; RESTRICTION ENZYMES; FUNCTIONAL-ANALYSIS; GENOME MODIFICATION;

D O I：

10.1007/s00018-016-2380-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Streptococcus-derived CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 (CRISPR-associated protein 9) system has emerged as a very powerful tool for targeted gene modifications in many living organisms including plants. Since the first application of this system for plant gene modification in 2013, this RNA-guided DNA endonuclease system has been extensively engineered to meet the requirements of functional genomics and crop trait improvement in a number of plant species. Given its short history, the emphasis of many studies has been the optimization of the technology to improve its reliability and efficiency to generate heritable gene modifications in plants. Here we review and analyze the features of customized CRISPR/Cas9 systems developed for plant genetic studies and crop breeding. We focus on two essential aspects: the heritability of gene modifications induced by CRISPR/Cas9 and the factors affecting its efficiency, and we provide strategies for future design of systems with improved activity and heritability in plants.

引用

页码：1075 / 1093

页数：19

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