Characterization of preptin-induced insulin secretion in pancreatic β-cells

被引：30

作者：

Cheng, Kai-Chun ^{[1
]}

Li, Ying-Xiao ^{[1
]}

Asakawa, Akihiro ^{[1
]}

Ushikai, Miharu ^{[1
]}

Kato, Ikuo ^{[2
]}

Sato, Yuki ^{[2
]}

Cheng, Juei-Tang ^{[3
]}

Inui, Akio ^{[1
]}

机构：

[1] Kagoshima Univ, Grad Sch Med & Dent Sci, Dept Psychosomat Internal Med, Kagoshima 8908520, Japan

[2] Hokuriku Univ, Fac Pharmaceut Sci, Dept Bioorgan Chem, Kanazawa, Ishikawa 9201180, Japan

[3] Chi Mei Med Ctr, Dept Med Res, Tainan 71004, Taiwan

来源：

JOURNAL OF ENDOCRINOLOGY | 2012年 / 215卷 / 01期

基金：

日本学术振兴会;

关键词：

CYTOPLASMIC FREE CALCIUM; CA2+ OSCILLATIONS; NEGATIVE FEEDBACK; RELEASE; DIACYLGLYCEROL; STIMULATION; VASOPRESSIN; MECHANISMS; INHIBITOR; RECEPTORS;

D O I：

10.1530/JOE-12-0176

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We aimed to characterize the effects of preptin on insulin secretion at the single-cell level, as well as the mechanisms underlying these changes, with respect to regulation by intracellular Ca2+ [Ca2+](i) mobilization. This study assessed the effect of preptin on insulin secretion and investigated the link between preptin and the phospholipase C (PLC)/protein kinase C (PKC) pathway at the cellular level using fura-2 pentakis(acetoxymethyl) ester-loaded insulin-producing cells (Min 6 cells). Our results demonstrate that preptin promotes insulin secretion in a concentration-dependent manner. Using a PLC inhibitor (chelerythrine) or a PKC inhibitor (U73122) resulted in a concentration-dependent decrease in insulin secretion. Also, preptin mixed with IGF2 receptor (IGF2R) antibodies suppressed insulin secretion in a dose-dependent manner, which indicates that activation of IGF2R is mediated probably because preptin is a type of proIGF2. In addition, preptin stimulated insulin secretion to a similar level as did glibenclamide. The activation of PKC/PLC by preptin stimulation is highly relevant to the potential mechanisms for increase in insulin secretion. Our results provide new insight into the insulin secretion of preptin, a secreted proIGF2-derived peptide that can induce greater efficacy of signal transduction resulting from PLC and PKC activation through the IGF2R. Journal of Endocrinology (2012) 215, 43-49

引用

页码：43 / 49

页数：7

共 29 条

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