Direct electrochemistry and superficial characterization of DNA-cytochrome c-MUA films on chemically modified gold surface

被引：20

作者：

Ding, XQ ^{[1
]}

Hu, JB ^{[1
]}

Li, QL ^{[1
]}

机构：

[1] Beijing Normal Univ, Dept Chem, Beijing 100875, Peoples R China

来源：

TALANTA | 2006年 / 68卷 / 03期

基金：

中国国家自然科学基金;

关键词：

electrochemistry; superficial characterization; cytochrome c; DNA; gold electrode;

D O I：

10.1016/j.talanta.2005.05.009

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Cytochrome c (Cyt. c) was immobilized on the 11-mercaptohendecanoic acid (MUA)-modified gold electrode. The electrode was stable and sensitive to Cyt. c. Later, DNA was also immobilized on the two-layer modified electrode. Cyclic voltammetry studies show that Cyt. c can interact with dsDNA and ssDNA. The binding site sizes were determined to be 15 base pairs per Cyt. c molecule with dsDNA and 30 nucleotides binding 1 Cyt. c molecule with ssDNA. The modified electrodes were characterized by quartz crystal microbalance (QCM), impedance spectroscopy and atomic force microscope (AFM). The modified electrode can be used for determining DNA. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：653 / 658

页数：6

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