Molecular cloning and chromosomal mapping of olfactory receptor genes expressed in the male germ line: Evidence for their wide distribution in the human genome

被引:44
作者
Vanderhaeghen, P
Schurmans, S
Vassart, G
Parmentier, M
机构
[1] FREE UNIV BRUSSELS,IRIBHN,B-1070 BRUSSELS,BELGIUM
[2] FREE UNIV BRUSSELS,DEPT MED GENET,B-1070 BRUSSELS,BELGIUM
关键词
D O I
10.1006/bbrc.1997.7043
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Olfactory receptor genes constitute the largest family of G protein-coupled receptors, We have previously shown that members of this family are expressed during spermatogenesis, and that the corresponding proteins are displayed on mature sperm cells. In each mammalian species, a restricted subset of olfactory receptors is expressed in male germ cells and displays a pattern of expression suggestive of their potential implication:in the control of sperm physiology. In addition to the cDNA fragments available previously, we now report the molecular cloning of two olfactory receptor cDNAs from a human testis library. Five olfactory receptor genes expressed in germ cells were localized in the human genome by radiation hybrid mapping. Three of the genes map to the short arm of chromosome 19 (19p13.1-19p31.3), one to chromosome 11 (11q22.1-22.3), and one to chromosome 17 (17q21-22), The former two localizations fall within clusters previously identified for members of the putative olfactory receptor gene family expressed in olfactory muco:sa. Similarly, sequence analysis has revealed that these testicular genes share no distinctive structural features from the other, non-testicular, members of the family. The expression of a subset of olfactory receptor genes in the male germ line is therefore not correlated to their belonging to a specific structural subgroup, or to a specific gene cluster or chromosomal segment. (C) 1997 Academic Press.
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页码:283 / 287
页数:5
相关论文
共 20 条
[1]   Genomic structure and transcription of a murine odorant receptor gene: Differential initiation of transcription in the olfactory and testicular cells [J].
Asai, H ;
Kasai, H ;
Matsuda, Y ;
Yamazaki, N ;
Nagawa, F ;
Sakano, H ;
Tsuboi, A .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 221 (02) :240-247
[2]   OLFACTORY RECEPTOR GENE-CLUSTER ON HUMAN-CHROMOSOME-17 - POSSIBLE DUPLICATION OF AN ANCESTRAL RECEPTOR REPERTOIRE [J].
BENARIE, N ;
LANCET, D ;
TAYLOR, C ;
KHEN, M ;
WALKER, N ;
LEDBETTER, DH ;
CARROZZO, R ;
PATEL, K ;
SHEER, D ;
LEHRACH, H ;
NORTH, MA .
HUMAN MOLECULAR GENETICS, 1994, 3 (02) :229-235
[3]   A NOVEL MULTIGENE FAMILY MAY ENCODE ODORANT RECEPTORS - A MOLECULAR-BASIS FOR ODOR RECOGNITION [J].
BUCK, L ;
AXEL, R .
CELL, 1991, 65 (01) :175-187
[4]  
CHAUDARY S, 1995, J BIOL CHEM, V270, P8755
[5]   ALLELIC INACTIVATION REGULATES OLFACTORY RECEPTOR GENE-EXPRESSION [J].
CHESS, A ;
SIMON, I ;
CEDAR, H ;
AXEL, R .
CELL, 1994, 78 (05) :823-834
[6]   OLFACTORY RECEPTOR-LIKE GENES ARE LOCATED IN THE HUMAN MAJOR HISTOCOMPATIBILITY COMPLEX [J].
FAN, WF ;
LIU, YC ;
PARIMOO, S ;
WEISSMAN, SM .
GENOMICS, 1995, 27 (01) :119-123
[7]   Sequence analysis in the olfactory receptor gene cluster on human chromosome 17: Recombinatorial events affecting receptor diversity [J].
Glusman, G ;
Clifton, S ;
Roe, B ;
Lancet, D .
GENOMICS, 1996, 37 (02) :147-160
[8]   AN STS-BASED MAP OF THE HUMAN GENOME [J].
HUDSON, TJ ;
STEIN, LD ;
GERETY, SS ;
MA, JL ;
CASTLE, AB ;
SILVA, J ;
SLONIM, DK ;
BAPTISTA, R ;
KRUGLYAK, L ;
XU, SH ;
HU, XT ;
COLBERT, AME ;
ROSENBERG, C ;
REEVEDALY, MP ;
ROZEN, S ;
HUI, L ;
WU, XY ;
VESTERGAARD, C ;
WILSON, KM ;
BAE, JS ;
MAITRA, S ;
GANIATSAS, S ;
EVANS, CA ;
DEANGELIS, MM ;
INGALLS, KA ;
NAHF, RW ;
HORTON, LT ;
ANDERSON, MO ;
COLLYMORE, AJ ;
YE, WJ ;
KOUYOUMJIAN, V ;
ZEMSTEVA, IS ;
TAM, J ;
DEVINE, R ;
COURTNEY, DF ;
RENAUD, MT ;
NGUYEN, H ;
OCONNOR, TJ ;
FIZAMES, C ;
FAURE, S ;
GYAPAY, G ;
DIB, C ;
MORISSETTE, J ;
ORLIN, JB ;
BIRREN, BW ;
GOODMAN, N ;
WEISSENBACH, J ;
HAWKINS, TL ;
FOOTE, S ;
PAGE, DC .
SCIENCE, 1995, 270 (5244) :1945-1954
[9]   EXPRESSION OF MEMBERS OF THE PUTATIVE OLFACTORY RECEPTOR GENE FAMILY IN MAMMALIAN GERM-CELLS [J].
PARMENTIER, M ;
LIBERT, F ;
SCHURMANS, S ;
SCHIFFMANN, S ;
LEFORT, A ;
EGGERICKX, D ;
LEDENT, C ;
MOLLEREAU, C ;
GERARD, C ;
PERRET, J ;
GROOTEGOED, A ;
VASSART, G .
NATURE, 1992, 355 (6359) :453-455
[10]  
PARMENTIER M, 1992, HDB RECEPTORS CHANNE, P237