Mid-trimester fetal livers are a rich source of CD34+/++ cells for transplantation

被引:34
作者
Golfier, F
Bárcena, A
Cruz, J
Harrison, M
Muench, MO
机构
[1] Univ Calif San Francisco, Fetal Treatment Ctr Res Lab, Bone Marrow Transplatat Lab, San Francisco, CA 94143 USA
[2] Univ Calif San Francisco, Dept Surg, Bone Marrow Transplatat Lab, San Francisco, CA 94143 USA
[3] Univ Calif San Francisco, Dept Mol Med, Bone Marrow Transplatat Lab, San Francisco, CA 94143 USA
关键词
fetal tissue transplantation; hematopoietic stem cells; liver; CD34(+) cells;
D O I
10.1038/sj.bmt.1701940
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Human fetal livers (FL), between 16 and 24 weeks of gestation, were studied for their potential as a source of hematopoietic stem cells for prenatal and postnatal transplantation. In this report we give a quantitative evaluation of human FL as a source of candidate stem cells, and develop a protocol for the isolation of these cells free of microbial contaminants and almost free of mature T cells. Human FLs contained a median 1.9 x 10(9) viable cells and a mean of 1.3 x 10(8) CD34(+/++) cells (range 1.1 x 10(7) to 4.7 x 10(8)). Regardless of gestational age, no significant differences mere apparent in the numbers of total progenitors or in the numbers of candidate stem cells (CD34(++) CD38(-) and CD34(++)CD(4+)), suggesting that the expansion in the liver of the early compartments of hematopoietic progenitors reaches a plateau after the sixteenth week of gestation. Colony-forming units culture (CFU-C) were found to range from 4.1 x 10(6) to 2.5 x 10(7) per FL. Positive selection of FL CD34(++) cells was achieved using the Baxter Isolex 50 device. An average purity of 74% and yield of 29% of CD34(+/++) cells was achieved. T cells were depleted by 99.95%, resulting in a mean of 6.5 x 10(3) T cells per processed liver. Analysis of candidate stem cell populations and primitive colony-forming cells (CFC) suggested a preferential enrichment of these cells over the total population of CD34(+/++) cells. Processed CD34(+/++) cells were found to be sterile. In conclusion, purification of FL progenitors between 16 and 24 weeks of gestation results in a large number of early progenitors suitable for irt utero and possibly postnatal transplantation.
引用
收藏
页码:451 / 461
页数:11
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