Human fetal livers (FL), between 16 and 24 weeks of gestation, were studied for their potential as a source of hematopoietic stem cells for prenatal and postnatal transplantation. In this report we give a quantitative evaluation of human FL as a source of candidate stem cells, and develop a protocol for the isolation of these cells free of microbial contaminants and almost free of mature T cells. Human FLs contained a median 1.9 x 10(9) viable cells and a mean of 1.3 x 10(8) CD34(+/++) cells (range 1.1 x 10(7) to 4.7 x 10(8)). Regardless of gestational age, no significant differences mere apparent in the numbers of total progenitors or in the numbers of candidate stem cells (CD34(++) CD38(-) and CD34(++)CD(4+)), suggesting that the expansion in the liver of the early compartments of hematopoietic progenitors reaches a plateau after the sixteenth week of gestation. Colony-forming units culture (CFU-C) were found to range from 4.1 x 10(6) to 2.5 x 10(7) per FL. Positive selection of FL CD34(++) cells was achieved using the Baxter Isolex 50 device. An average purity of 74% and yield of 29% of CD34(+/++) cells was achieved. T cells were depleted by 99.95%, resulting in a mean of 6.5 x 10(3) T cells per processed liver. Analysis of candidate stem cell populations and primitive colony-forming cells (CFC) suggested a preferential enrichment of these cells over the total population of CD34(+/++) cells. Processed CD34(+/++) cells were found to be sterile. In conclusion, purification of FL progenitors between 16 and 24 weeks of gestation results in a large number of early progenitors suitable for irt utero and possibly postnatal transplantation.
机构:
COLL FRANCE, CNRS, INST EMBRYOL CELLULAIRE & MOLEC, NOGENT SUR MARNE, FRANCECOLL FRANCE, CNRS, INST EMBRYOL CELLULAIRE & MOLEC, NOGENT SUR MARNE, FRANCE
Charbord, P
Tavian, M
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COLL FRANCE, CNRS, INST EMBRYOL CELLULAIRE & MOLEC, NOGENT SUR MARNE, FRANCECOLL FRANCE, CNRS, INST EMBRYOL CELLULAIRE & MOLEC, NOGENT SUR MARNE, FRANCE
Tavian, M
Humeau, L
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COLL FRANCE, CNRS, INST EMBRYOL CELLULAIRE & MOLEC, NOGENT SUR MARNE, FRANCECOLL FRANCE, CNRS, INST EMBRYOL CELLULAIRE & MOLEC, NOGENT SUR MARNE, FRANCE
Humeau, L
Peault, B
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COLL FRANCE, CNRS, INST EMBRYOL CELLULAIRE & MOLEC, NOGENT SUR MARNE, FRANCECOLL FRANCE, CNRS, INST EMBRYOL CELLULAIRE & MOLEC, NOGENT SUR MARNE, FRANCE
机构:
COLL FRANCE, CNRS, INST EMBRYOL CELLULAIRE & MOLEC, NOGENT SUR MARNE, FRANCECOLL FRANCE, CNRS, INST EMBRYOL CELLULAIRE & MOLEC, NOGENT SUR MARNE, FRANCE
Charbord, P
Tavian, M
论文数: 0引用数: 0
h-index: 0
机构:
COLL FRANCE, CNRS, INST EMBRYOL CELLULAIRE & MOLEC, NOGENT SUR MARNE, FRANCECOLL FRANCE, CNRS, INST EMBRYOL CELLULAIRE & MOLEC, NOGENT SUR MARNE, FRANCE
Tavian, M
Humeau, L
论文数: 0引用数: 0
h-index: 0
机构:
COLL FRANCE, CNRS, INST EMBRYOL CELLULAIRE & MOLEC, NOGENT SUR MARNE, FRANCECOLL FRANCE, CNRS, INST EMBRYOL CELLULAIRE & MOLEC, NOGENT SUR MARNE, FRANCE
Humeau, L
Peault, B
论文数: 0引用数: 0
h-index: 0
机构:
COLL FRANCE, CNRS, INST EMBRYOL CELLULAIRE & MOLEC, NOGENT SUR MARNE, FRANCECOLL FRANCE, CNRS, INST EMBRYOL CELLULAIRE & MOLEC, NOGENT SUR MARNE, FRANCE