POSH, a scaffold protein for JNK signaling, binds to ALG-2 and ALIX in Drosophila

被引：39

作者：

Tsuda, Manabu

Seong, Ki-Hyeon

Aigaki, Toshiro

机构：

[1] Tokyo Metropolitan Univ, Dept Biol Sci, Hachioji, Tokyo 1920397, Japan

[2] Japan Biol Informat Consortium, Tokyo 1358073, Japan

[3] RIKEN, Tsukuba Inst, Mol Genet Lab, Tsukuba, Ibaraki 3050074, Japan

来源：

FEBS LETTERS | 2006年 / 580卷 / 13期

基金：

日本学术振兴会;

关键词：

plenty of SH3s; apoptosis-linked gene-2; ALG-2-interacting protein X; c-Jun N-terminal kinase; cell death;

D O I：

10.1016/j.febslet.2006.05.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plenty of SH3s (POSH) functions as a scaffold protein for the Jun N-terminal kinase (JNK) signal transduction pathway, which leads to cell death in mammalian cultured cells and Drosophila. Here, we show that POSH forms a complex with Apoptosis-linked gene-2 (ALG-2) and ALG-2-interacting protein (ALIX/AIP1) in a calcium-dependent manner. Overexpression of ALG-2 or ALIX in developing imaginal eye discs resulted in roughened or melanized eyes, respectively. These phenotypes were enhanced by co-overexpression of POSH. We found that overexpression of either gene could induce ectopic JNK activation, suggesting that POSH/ALG-2/ALIX may function together in the regulation of the JNK pathway. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

收藏

页码：3296 / 3300

页数：5

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