Activation of glycolysis in human muscle in vivo

被引:88
作者
Conley, KE
Blei, ML
Richards, TL
Kushmerick, MJ
Jubrias, SA
机构
[1] UNIV WASHINGTON, MED CTR, DEPT BIOENGN, SEATTLE, WA 98195 USA
[2] UNIV WASHINGTON, MED CTR, DEPT PHYSIOL & BIOPHYS, SEATTLE, WA 98195 USA
[3] UNIV WASHINGTON, MED CTR, DEPT REHABIL MED, SEATTLE, WA 98195 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1997年 / 273卷 / 01期
关键词
high-energy phosphate; phosphorus-31 magnetic resonance spectroscopy; adenosine 5'-triphosphate; phosphocreatine; muscle metabolism;
D O I
10.1152/ajpcell.1997.273.1.C306
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We tested the cytoplasmic control mechanisms for glycolytic ATP synthesis in human wrist flexor muscles. The forearm was made ischemic and activated by maximal twitch stimulation of the median and ulnar nerves in 10 subjects. Kinetic changes in phosphocreatine, P-i, ADP, ATP, sugar phosphates, and pH were measured by P-31 magnetic resonance spectroscopy at 7.1-s intervals. Proton production was determined from pH and tissue buffer capacity during stimulation. Glycolysis was activated between 30 and 50 stimulations, and the rate did not significantly change through the stimulation period. The independence of glycolytic rate on [P-i], [ADP], or [AMP] indicates that feedback regulation by these metabolites could not account for this activation of glycolysis. However, glycolytic H+ and ATP production increased sixfold from 0.5 to 3 Hz, indicating that glycolytic rate reflected muscle activation frequency. This dependence of glycolytic rate on muscle stimulation frequency and independence on metabolite levels is consistent with control of glycolysis by Ca2+.
引用
收藏
页码:C306 / C315
页数:10
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