Novel detection system for biomolecules using nano-sized bacterial magnetic particles and magnetic force microscopy

被引：51

作者：

Amemiya, Y ^{[1
]}

Tanaka, T ^{[1
]}

Yoza, B ^{[1
]}

Matsunaga, T ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol, Koganei, Tokyo 1848588, Japan

来源：

JOURNAL OF BIOTECHNOLOGY | 2005年 / 120卷 / 03期

关键词：

D O I：

10.1016/j.jbiotec.2005.06.028

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A system for streptavidin detection using biotin conjugated to nano-sized bacterial magnetic particles (BMPs) has been developed. BMPs, isolated from magnetic bacteria, were used as magnetic markers for magnetic force microscopy (MFM) imaging. The magnetic signal was obtained from a single particle using MFM without application of an external magnetic field. The number of biotin conjugated BMPs (biotin-BMPs) bound to streptavidin immobilized on the glass slides increased with streptavidin concentrations up to 100 pg/ml. The minimum streptavidin detection limit using this technique is 1 pg/ml, which is 100 times more sensitive than a conventional fluorescent detection system. This is the first report using single domain nano-sized magnetic particles as magnetic markers for biosensing. This assay system can be used for immunoassay and DNA detection with high sensitivities. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：308 / 314

页数：7

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