Engineering of an Escherichia coli strain for the production of 3-methyl-1-butanol

被引：119

作者：

Connor, Michael R. ^{[1
]}

Liao, James C. ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 18期

关键词：

D O I：

10.1128/AEM.00468-08

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

3-Methyl-1-butanol is a potential fuel additive or substitute. Previously this compound was identified in small quantities in yeast fermentation as one of the fusel alcohols. In this work, we engineered an Escherichia coli strain to produce 3-methyl-1-butanol from glucose via the host's amino acid biosynthetic pathways. Strain improvement with the removal of feedback inhibition and competing pathways increased the selectivity and productivity of 3-methyl-1-butanol. This work demonstrates the feasibility of production of 3-methyl-1-butanol as a biofuel and shows promise in using E. coli as a host for production.

引用

页码：5769 / 5775

页数：7

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