The distances from tyrosine D to redox-active components on the donor side of Photosystem II determined by pulsed electron-electron double resonance

被引：48

作者：

Hara, H

Kawamori, A

Astashkin, AV

Ono, T

机构：

[1] KWANSEI GAKUIN UNIV,FAC SCI,NISHINOMIYA,HYOGO 662,JAPAN

[2] INST PHYS & CHEM RES,WAKO,SAITAMA 35101,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1996年 / 1276卷 / 02期

关键词：

calcium ion-depleted Photosystem II; manganese-cluster; multiline signal; S-3; signal; electron spin echo; electron-electron double resonance;

D O I：

10.1016/0005-2728(96)00071-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A pulsed electron-electron double resonance (ELDOR) method was applied to measure the dipole interactions between paramagnetic species on the donor side of Photosystem II. The distance between the Mn cluster and the redox-active tyrosine residue Y-D was determined to be 27 +/- 0.2 Angstrom in the S-2 state of the oxygen-evolving Photosystem II. In Ca2+-depleted preparations the same distance was obtained from the measurement of the modified multiline signal. The distance between Y-D and the paramagnetic species giving. rise to the split S-3 signal was found to be 30 +/- 0.2 Angstrom.

引用

收藏

页码：140 / 146

页数：7

相关论文

共 27 条

[1] DISTANCE BETWEEN TYROSINES Z(+) AND D+ IN PLANT PHOTOSYSTEM-II AS DETERMINED BY PULSED EPR [J].

ASTASHKIN, AV ;

KODERA, Y ;

KAWAMORI, A .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1994, 1187 (01) :89-93

[2] A HIGHLY RESOLVED, OXYGEN-EVOLVING PHOTOSYSTEM-II PREPARATION FROM SPINACH THYLAKOID MEMBRANES - ELECTRON-PARAMAGNETIC-RES AND ELECTRON-TRANSPORT PROPERTIES [J].

BERTHOLD, DA ;

BABCOCK, GT ;

YOCUM, CF .

FEBS LETTERS, 1981, 134 (02) :231-234

[3] NATURE OF THE INHIBITION OF THE OXYGEN-EVOLVING ENZYME OF PHOTOSYSTEM-II INDUCED BY NACL WASHING AND REVERSED BY THE ADDITION OF CA-2+ OR SR-2+ [J].

BOUSSAC, A ;

RUTHERFORD, AW .

BIOCHEMISTRY, 1988, 27 (09) :3476-3483

[4] HISTIDINE OXIDATION IN THE OXYGEN-EVOLVING PHOTOSYSTEM-II ENZYME [J].

BOUSSAC, A ;

ZIMMERMANN, JL ;

RUTHERFORD, AW ;

LAVERGNE, J .

NATURE, 1990, 347 (6290) :303-306

[5] SITE-DIRECTED MUTAGENESIS IDENTIFIES A TYROSINE RADICAL INVOLVED IN THE PHOTOSYNTHETIC OXYGEN-EVOLVING SYSTEM [J].

DEBUS, RJ ;

BARRY, BA ;

BABCOCK, GT ;

MCINTOSH, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (02) :427-430

[6] THE MANGANESE AND CALCIUM-IONS OF PHOTOSYNTHETIC OXYGEN EVOLUTION [J].

DEBUS, RJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1102 (03) :269-352

[7] INTERMEDIATES OF A POLYNUCLEAR MANGANESE CENTER INVOLVED IN PHOTOSYNTHETIC OXIDATION OF WATER [J].

DISMUKES, GC ;

SIDERER, Y .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (01) :274-278

[8] EPR RELAXATION MEASUREMENTS OF PHOTOSYSTEM-II REACTION CENTERS - INFLUENCE OF S-STATE OXIDATION AND TEMPERATURE [J].

EVELO, RG ;

STYRING, S ;

RUTHERFORD, AW ;

HOFF, AJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 973 (03) :428-442

[9]

GILCHRIST ML, 1995, IN PRESS P NATL ACAD

[10] USING SATURATION RECOVERY EPR TO MEASURE DISTANCES IN PROTEINS - APPLICATIONS TO PHOTOSYSTEM-II [J].

HIRSH, DJ ;

BECK, WF ;

INNES, JB ;

BRUDVIG, GW .

BIOCHEMISTRY, 1992, 31 (02) :532-541