Phosphoinositides Regulate P2X4 ATP-Gated Channels through Direct Interactions

被引:77
作者
Bernier, Louis-Philippe [1 ]
Ase, Ariel R. [1 ]
Chevallier, Stephanie [1 ]
Blais, Dominique [1 ]
Zhao, Qi [2 ]
Boue-Grabot, Eric [3 ]
Logothetis, Diomedes [2 ]
Seguela, Philippe [1 ]
机构
[1] McGill Univ, Montreal Neurol Inst, Dept Neurol & Neurosurg, Montreal, PQ H3A 2B4, Canada
[2] NYU, Mt Sinai Sch Med, Dept Struct & Chem Biol, New York, NY 10029 USA
[3] Univ Bordeaux 2, CNRS, UMR 5227, F-33076 Bordeaux, France
基金
美国国家卫生研究院; 加拿大健康研究院;
关键词
P2X; purinergic; PIP2; calcium; microglia; neuropathic pain;
D O I
10.1523/JNEUROSCI.3038-08.2008
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
P2X receptors are ATP-gated nonselective cation channels highly permeable to calcium that contribute to nociception and inflammatory responses. The P2X(4) subtype, upregulated in activated microglia, is thought to play a critical role in the development of tactile allodynia following peripheral nerve injury. Posttranslational regulation of P2X(4) function is crucial to the cellular mechanisms of neuropathic pain, however it remains poorly understood. Here, we show that the phosphoinositides PI(4,5)P-2 (PIP2) and PI(3,4,5)P-3 (PIP3), products of phosphorylation by wortmannin-sensitive phosphatidylinositol 4-kinases and phosphatidylinositol 3-kinases, can modulate the function of native and recombinant P2X(4) receptor channels. In BV-2 microglial cells, depleting the intracellular levels of PIP2 and PIP3 with wortmannin significantly decreased P2X(4) current amplitude and P2X(4)-mediated calcium entry measured in patch clamp recordings and ratiometric ion imaging, respectively. Wortmannin-induced depletion of phosphoinositides in Xenopus oocytes decreased the current amplitude of P2X(4) responses by converting ATP into a partial agonist. It also decreased their recovery from desensitization and affected their kinetics. Injection of phosphoinositides in wortmannin-treated oocytes reversed these effects and application of PIP2 on excised inside-out macropatches rescued P2X(4) currents from rundown. Moreover, we report the direct interaction of phospholipids with the proximal C-terminal domain of P2X(4) subunit (Cys(360)-Val(375)) using an in vitro binding assay. These results demonstrate novel regulatory roles of the major signaling phosphoinositides PIP2 and PIP3 on P2X(4) function through direct channel-lipid interactions.
引用
收藏
页码:12938 / 12945
页数:8
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