Carbon-14-urea breath test as a noninvasive method to monitor Helicobacter felis colonization in mice

被引：11

作者：

Glauser, M ^{[1
]}

Michetti, P ^{[1
]}

Blum, AL ^{[1
]}

CorthesyTheulaz, I ^{[1
]}

机构：

[1] CHU VAUDOIS, DIV GASTROENTEROL, CH-1011 LAUSANNE, SWITZERLAND

来源：

DIGESTION | 1996年 / 57卷 / 01期

关键词：

Helicobacter felis infection; C-14-urea breath test in mice; method;

D O I：

10.1159/000201309

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

We have developed a C-14-urea breath test to follow the course of Helicobacter felis infection in mice. Peak (CO2)-C-14 production occurred approximately 8 min after substrate administration. The test values were compared to those from a rapid urease test and correlated with the presence of pathogens by histology. The sensitivity was 99%, specificity 91%, positive predictive value 95% and negative predictive value 99% when the assay was conducted in fasted mice. We conclude that in mice the breath test analysis is a useful noninvasive method for detecting the presence of H. felis or for evaluating therapeutic agents affecting growth or survival of the organism.

引用

页码：30 / 34

页数：5

共 14 条

[1]

ABT AF, 1966, B JOHNS HOPKINS HOSP, V119, P316

[2] IMMUNIZATION AGAINST GASTRIC HELICOBACTER INFECTION IN A MOUSE HELICOBACTER-FELIS MODEL [J].

CHEN, MH ;

LEE, A ;

HAZELL, S .

LANCET, 1992, 339 (8801) :1120-1121

[3] IMMUNIZATION AGAINST GASTRIC INFECTION WITH HELICOBACTER SPECIES - 1ST STEP IN THE PROPHYLAXIS OF GASTRIC-CANCER [J].

CHEN, MH ;

LEE, A ;

HAZELL, S ;

HU, PJ ;

LI, YY .

ZENTRALBLATT FUR BAKTERIOLOGIE-INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY VIROLOGY PARASITOLOGY AND INFECTIOUS DISEASES, 1993, 280 (1-2) :155-165

[4] ORAL IMMUNIZATION AGAINST HELICOBACTER-PYLORI [J].

CZINN, SJ ;

NEDRUD, JG .

INFECTION AND IMMUNITY, 1991, 59 (07) :2359-2363

[5]

GRAHAM DY, 1987, LANCET, V1, P1174

[6]

GUGLIELMETTI P, 1991, MICROBIOLOGICA, V14, P131

[7]

HEGEDUS ED, 1991, SCAND J GASTEROENTER, V26, P909

[8] A SMALL ANIMAL-MODEL OF HUMAN HELICOBACTER-PYLORI ACTIVE CHRONIC GASTRITIS [J].

LEE, A ;

FOX, JG ;

OTTO, G ;

MURPHY, J .

GASTROENTEROLOGY, 1990, 99 (05) :1315-1323

[9]

MARSHALL BJ, 1988, J NUCL MED, V29, P11

[10] DEVELOPMENT OF A C-14 UREA BREATH TEST IN FERRETS COLONIZED WITH HELICOBACTER-MUSTELAE - EFFECTS OF TREATMENT WITH BISMUTH, ANTIBIOTICS, AND UREASE INHIBITORS [J].

MCCOLM, AA ;

BAGSHAW, JA ;

OMALLEY, CF .

GUT, 1993, 34 (02) :181-186

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