Specific identification of Pasteurella multocida serogroup-A isolates by PCR assay

被引：20

作者：

Gautam, R ^{[1
]}

Kumar, AA ^{[1
]}

Singh, VP ^{[1
]}

Dutta, TK ^{[1
]}

Shivachandra, SB ^{[1
]}

机构：

[1] Indian Vet Res Inst, Div Bacteriol & Mycol, Izatnagar 243122, Uttar Pradesh, India

来源：

RESEARCH IN VETERINARY SCIENCE | 2004年 / 76卷 / 03期

关键词：

Pasteurella multocida; Serogroup A; PCR;

D O I：

10.1016/j.rvsc.2003.10.005

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A polymerase chain reaction (PCR) assay targeting the hyaC-hyaD gene was developed and used to identify strains of Pasteurella multocida belonging to serogroup-A. A set of serogroup-specific-PCR primers amplified a 564 bp product from genomic DNA prepared from bacterial cells or directly from bacterial colonies. This method detected as low as 10 ng of bacterial DNA and had a specificity of 100% for P. multocida serogroup-A. A nested PCR method yielded a single 374 bp product. All fifty isolates were also shown to be identical by restriction fragment length polymorphism (RFLP) analysis of the PCR products after digestion with Bg/II. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：179 / 185

页数：7

共 13 条

[1]

Blackall PJ, 2000, AVIAN PATHOL, V29, P271, DOI 10.1080/03079450050118395

[2] The capsule is a virulence determinant in the pathogenesis of Pasteurella multocida M1404 (B:2) [J].

Boyce, JD ;

Adler, B .

INFECTION AND IMMUNITY, 2000, 68 (06) :3463-3468

[3]

CARTER GR, 1955, AM J VET RES, V16, P481

[4] Enhanced detection of toxigenic Pasteurella multocida directly from nasal swabs using a nested polymerase chain reaction [J].

Choi, C ;

Chae, C .

VETERINARY JOURNAL, 2001, 162 (03) :255-258

[5]

Chung JY, 1998, FEMS MICROBIOL LETT, V166, P289, DOI 10.1111/j.1574-6968.1998.tb13903.x

[6] IMMUNOGENS OF PASTEURELLA [J].

CONFER, AW .

VETERINARY MICROBIOLOGY, 1993, 37 (3-4) :353-368

[7]

DELAWIS MCL, 1996, INT WORKSH DIAGN CON, P28

[8] Pasteurella multocida subsp multocida and P. multocida subsp septica -: Differentiation by PCR fingerprinting and α-glucosidase activity [J].

Gerardo, SH ;

Citron, DM ;

Claros, MC ;

Fernandez, HT ;

Goldstein, EJC .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (07) :2558-2564

[9] SPECIFIC AMPLIFICATION WITH PCR OF A REFRACTORY SEGMENT OF GENOMIC DNA [J].

HAQQI, TM ;

SARKAR, G ;

DAVID, CS ;

SOMMER, SS .

NUCLEIC ACIDS RESEARCH, 1988, 16 (24) :11844-11844

[10] FOWL CHOLERA - GEL-DIFFUSION PRECIPITIN TEST FOR SEROTYPING PASTEURELLA-MULTOCIDA FROM AVIAN SPECIES [J].

HEDDLESTON, KL ;

REBERS, PA ;

GALLAGHER, JE .

AVIAN DISEASES, 1972, 16 (04) :925-+

← 1 2 →