Combination of immunohistochemistry and laser ablation ICP mass spectrometry for imaging of cancer biomarkers

被引：91

作者：

Seuma, Jaume ^{[1
]}

Bunch, Josephine ^{[1
]}

Cox, Alan ^{[1
]}

McLeod, Cameron ^{[1
]}

Bell, Jane ^{[2
]}

Murray, Cliff ^{[2
]}

机构：

[1] Univ Sheffield, Ctr Analyt Sci, Dept Chem, Sheffield S3 7HF, S Yorkshire, England

[2] Med Solut, Nottingham, England

来源：

PROTEOMICS | 2008年 / 8卷 / 18期

关键词：

cancer biomarkers; cellular imaging; elemental (Au/Ag) tagged antibodies; HER2; laser ablation ICP mass spectrometry;

D O I：

10.1002/pmic.200800167

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Laser ablation (LA) ICP-MS has been developed as a new tool for imaging of cancer biomarkers in tissue sections. The distribution of two breast cancer-associated proteins, MUC-1 and HER2 was studied based on multiple line rastering of tissue sections and measurement of relevant Au/Ag tagged antibodies bound to the tissue. Comparisons with optical microscopy indicated extremely high sensitivity for the LA technique and sufficiently good resolution to permit fine scale feature mapping at the cellular level. Application to the quantitative assessment of HER2 expression in tissue microarrays was demonstrated.

引用

页码：3775 / 3784

页数：10

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