The effect of insulin on Δ5 desaturation in hepG2 human hepatoma cells and L6 rat muscle myoblasts

被引：7

作者：

Loizou, CL ^{[1
]}

Ozanne, SE ^{[1
]}

Hales, CN ^{[1
]}

机构：

[1] Univ Cambridge, Addenbrookes Hosp, Dept Clin Biochem, Cambridge CB2 2QR, England

来源：

PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 1999年 / 61卷 / 02期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1054/plef.1999.0076

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In humans there is a correlation between the ratio of arachidonic acid (20:4n-6) to cis 8,11,14 eicosatrienoic acid (20:3n-6) in skeletal muscle phospholipids and insulin sensitivity. This has been interpreted as indicating a link between the activity of the Delta 5 desaturase enzyme and muscle insulin sensitivity. The present study addressed the possibility that insulin regulates Delta 5 desaturase activity using L6 rat myoblasts and hepG2 human hepatoma cells. Both cell lines responded to insulin by increasing the amount of D-[U-C-14] glucose incorporated into glycogen. In L6 cells, insulin stimulated cis 8,11,14 eicosatrienoic acid uptake and arachidonic acid production but had no effect on the percentage conversion of cis 8,11,14 eicosatrienoic acid to arachidonic acid. In hepG2 cells, insulin had no effect on cis 8,11,14 eicosatrienoic acid uptake or arachidonic acid production. These results suggest that insulin has no direct effect on Delta 5 desaturase activity in the liver but can alter arachidonic acid production in muscle by altering substrate availability.

引用

页码：89 / 95

页数：7

共 42 条

[1]

ANGLETTI C, 1995, MOL CELL BIOCHEM, V143, P99

[2]

ASHWELL M, 1993, UNSATURATED FATTY AC, P35

[3]

BAKER C, 1998, DIABETIC MED S1, V15, pS29

[4]

BAYONA KYW, 1995, J BIOL CHEM, V13, P23975

[5] THE METABOLISM AND AVAILABILITY OF ESSENTIAL FATTY-ACIDS IN ANIMAL AND HUMAN TISSUES [J].

BEZARD, J ;

BLOND, JP ;

BERNARD, A ;

CLOUET, P .

REPRODUCTION NUTRITION DEVELOPMENT, 1994, 34 (06) :539-568

[6] THE RELATION BETWEEN INSULIN SENSITIVITY AND THE FATTY-ACID COMPOSITION OF SKELETAL-MUSCLE PHOSPHOLIPIDS [J].

BORKMAN, M ;

STORLIEN, LH ;

PAN, DA ;

JENKINS, AB ;

CHISHOLM, DJ ;

CAMPBELL, LV .

NEW ENGLAND JOURNAL OF MEDICINE, 1993, 328 (04) :238-244

[7]

CASTUMA JC, 1972, J LIPID RES, V13, P783

[8] Adrenic acid Δ4 desaturation and fatty acid composition in the liver of marine-oil fed streptozotocin diabetic rats [J].

Chanussot, B ;

Asdrubal, P ;

Huang, YS ;

Poisson, JP .

PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, 1997, 57 (06) :539-544

[9] EFFECTS OF DIETARY FATS ON FATTY-ACID COMPOSITION AND DELTA-5 DESATURASE IN NORMAL AND DIABETIC RATS [J].

DANG, AQ ;

KEMP, K ;

FAAS, FH ;

CARTER, WJ .

LIPIDS, 1989, 24 (10) :882-889

[10] GLUCOCORTICOID AND MINERALOCORTICOID HORMONES DEPRESS LIVER DELTA-5 DESATURASE ACTIVITY THROUGH DIFFERENT MECHANISMS [J].

DEALANIZ, MJT ;

MARRA, CA .

LIPIDS, 1992, 27 (08) :599-604

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