DNA vaccine for hepatitis B: Evidence for immunogenicity in chimpanzees and comparison with other vaccines

被引：193

作者：

Davis, HL

McCluskie, MJ

Gerin, JL

Purcell, RH

机构：

[1] UNIV OTTAWA,FAC HLTH SCI,PROGRAM PHYSIOTHERAPY,OTTAWA,ON K1H 8M5,CANADA

[2] UNIV OTTAWA,FAC MED,DEPT PHYSIOL,OTTAWA,ON K1H 8M5,CANADA

[3] GEORGETOWN UNIV,MED CTR,DIV MOL VIROL & IMMUNOL,ROCKVILLE,MD 20852

[4] NIAID,INFECT DIS LAB,NIH,BETHESDA,MD 20892

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1996年 / 93卷 / 14期

关键词：

D O I：

10.1073/pnas.93.14.7213

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Vaccination of two chimpanzees against hepatitis B virus (HBV) by intramuscular injection of plasmid DNA encoding the major and middle HBV envelope proteins induced group-, subtype- and preS2-specific antibodies. These were initially of IgM isotype, and then they were of IgG (predominantly IgG1) isotype, The chimpanzee injected with 2 mg of DNA attained >100 milli-international units/ml of anti-HBs antibody after one injection and 14,000 milli-international units/ml after four injections. A smaller dose (400 mu g) induced lower and transient titers, but a strong anamnestic response occurred 1 year later. Comparison with responses in 23 chimpanzees receiving various antigen-based HBV vaccines suggests that the DNA approach is promising for prophylactic immunization against HBV.

引用

页码：7213 / 7218

页数：6

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