Exosome-like vesicles with dipeptidyl peptidase IV in human saliva

被引:207
作者
Ogawa, Yuko [1 ]
Kanai-Azuma, Masami [2 ]
Akimoto, Yoshihiro [2 ]
Kawakami, Hayato [2 ]
Yanoshita, Ryohel [1 ]
机构
[1] Hoshi Univ, Inst Med Chem, Shinagawa Ku, Tokyo 1428501, Japan
[2] Kyorin Univ, Sch Med, Dept Anat, Tokyo 1818611, Japan
关键词
exosome; human whole saliva; dipeptidyl peptidase IV; galectin-3; substance P;
D O I
10.1248/bpb.31.1059
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Saliva contains a large number of proteins that participate in the protection of oral tissue. We found, for the first time, small vesicles (30-130 nm in diameter) in human whole saliva. Vesicles from saliva were identified by electron microscopy after isolation by gel-filtration on Sepharose CL-4B. They resemble exosomes, which are vesicles with an endosome-derived limiting membrane that are secreted by a diverse range of cell types. We performed a biochemical characterization of these vesicles by amino acid sequence analysis and Western blot analysis. We found that they contain dipeptidyl peptidase IV (DPP IV), galectin-3 and immunoglobulin A, which have potential to influence immune response. The DPP IV in the vesicles was metabolically active in cleaving substance P and glucose-dependent insulinotropic polypeptide to release N-terminal dipeptides. Our results demonstrate that human whole saliva contains exosome-like vesicles; they might participate in the catabolism of bioactive peptides and play a regulatory role in local immune defense in the oral cavity.
引用
收藏
页码:1059 / 1062
页数:4
相关论文
共 38 条
[1]   Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid [J].
Admyre, C ;
Grunewald, J ;
Thyberg, J ;
Gripenbäck, S ;
Tornling, G ;
Eklund, A ;
Scheynius, A ;
Gabrielsson, S .
EUROPEAN RESPIRATORY JOURNAL, 2003, 22 (04) :578-583
[2]   Localization of the O-linked N-acetylglucosamine transferase in rat pancreas [J].
Akimoto, Y ;
Kreppel, LK ;
Hirano, H ;
Hart, GW .
DIABETES, 1999, 48 (12) :2407-2413
[3]   Salivary proteins: Protective and diagnostic value in cariology? [J].
Amerongen, AV ;
Bolscher, JGM ;
Veerman, ECI .
CARIES RESEARCH, 2004, 38 (03) :247-253
[4]  
Cuello C, 1998, BRIT J RHEUMATOL, V37, P779
[5]   Follicular dendritic cells carry MHC class II-expressing microvesicles at their surface [J].
Denzer, K ;
van Eijk, M ;
Kleijmeer, MJ ;
Jakobson, E ;
de Groot, C ;
Geuze, HJ .
JOURNAL OF IMMUNOLOGY, 2000, 165 (03) :1259-1265
[6]   Alanine aminopeptidase and dipeptidylpeptidase IV in Saliva:: the possible role in periodontal disease [J].
Elgün, S ;
Özmeriç, N ;
Demirtas, S .
CLINICA CHIMICA ACTA, 2000, 298 (1-2) :187-191
[7]   Identification, proteomic profiling, and origin of ram epididymal fluid exosome-like vesicles [J].
Gatti, JL ;
Métayer, S ;
Belghazi, M ;
Dacheux, F ;
Dacheux, JL .
BIOLOGY OF REPRODUCTION, 2005, 72 (06) :1452-1465
[8]   Dipeptidyl peptidase IV from human serum: Purification, characterization, and N-terminal amino acid sequence [J].
Iwaki-Egawa, S ;
Watanabe, Y ;
Kikuya, Y ;
Fujimoto, Y .
JOURNAL OF BIOCHEMISTRY, 1998, 124 (02) :428-433
[9]   Exosomes biological significance: A concise review [J].
Johnstone, RM .
BLOOD CELLS MOLECULES AND DISEASES, 2006, 36 (02) :315-321
[10]   The polymeric immunoglobulin receptor: bridging innate and adaptive immune responses at mucosal surfaces [J].
Kaetzel, CS .
IMMUNOLOGICAL REVIEWS, 2005, 206 :83-99