exosome;
human whole saliva;
dipeptidyl peptidase IV;
galectin-3;
substance P;
D O I:
10.1248/bpb.31.1059
中图分类号:
R9 [药学];
学科分类号:
1007 ;
摘要:
Saliva contains a large number of proteins that participate in the protection of oral tissue. We found, for the first time, small vesicles (30-130 nm in diameter) in human whole saliva. Vesicles from saliva were identified by electron microscopy after isolation by gel-filtration on Sepharose CL-4B. They resemble exosomes, which are vesicles with an endosome-derived limiting membrane that are secreted by a diverse range of cell types. We performed a biochemical characterization of these vesicles by amino acid sequence analysis and Western blot analysis. We found that they contain dipeptidyl peptidase IV (DPP IV), galectin-3 and immunoglobulin A, which have potential to influence immune response. The DPP IV in the vesicles was metabolically active in cleaving substance P and glucose-dependent insulinotropic polypeptide to release N-terminal dipeptides. Our results demonstrate that human whole saliva contains exosome-like vesicles; they might participate in the catabolism of bioactive peptides and play a regulatory role in local immune defense in the oral cavity.
机构:
Karolinska Hosp & Inst, Unit Clin Allergy Res, Dept Med, Stockholm, SwedenKarolinska Hosp & Inst, Unit Clin Allergy Res, Dept Med, Stockholm, Sweden
Admyre, C
;
Grunewald, J
论文数: 0引用数: 0
h-index: 0
机构:Karolinska Hosp & Inst, Unit Clin Allergy Res, Dept Med, Stockholm, Sweden
Grunewald, J
;
Thyberg, J
论文数: 0引用数: 0
h-index: 0
机构:Karolinska Hosp & Inst, Unit Clin Allergy Res, Dept Med, Stockholm, Sweden
Thyberg, J
;
Gripenbäck, S
论文数: 0引用数: 0
h-index: 0
机构:Karolinska Hosp & Inst, Unit Clin Allergy Res, Dept Med, Stockholm, Sweden
Gripenbäck, S
;
Tornling, G
论文数: 0引用数: 0
h-index: 0
机构:Karolinska Hosp & Inst, Unit Clin Allergy Res, Dept Med, Stockholm, Sweden
Tornling, G
;
Eklund, A
论文数: 0引用数: 0
h-index: 0
机构:Karolinska Hosp & Inst, Unit Clin Allergy Res, Dept Med, Stockholm, Sweden
Eklund, A
;
Scheynius, A
论文数: 0引用数: 0
h-index: 0
机构:Karolinska Hosp & Inst, Unit Clin Allergy Res, Dept Med, Stockholm, Sweden
Scheynius, A
;
Gabrielsson, S
论文数: 0引用数: 0
h-index: 0
机构:Karolinska Hosp & Inst, Unit Clin Allergy Res, Dept Med, Stockholm, Sweden
机构:
Karolinska Hosp & Inst, Unit Clin Allergy Res, Dept Med, Stockholm, SwedenKarolinska Hosp & Inst, Unit Clin Allergy Res, Dept Med, Stockholm, Sweden
Admyre, C
;
Grunewald, J
论文数: 0引用数: 0
h-index: 0
机构:Karolinska Hosp & Inst, Unit Clin Allergy Res, Dept Med, Stockholm, Sweden
Grunewald, J
;
Thyberg, J
论文数: 0引用数: 0
h-index: 0
机构:Karolinska Hosp & Inst, Unit Clin Allergy Res, Dept Med, Stockholm, Sweden
Thyberg, J
;
Gripenbäck, S
论文数: 0引用数: 0
h-index: 0
机构:Karolinska Hosp & Inst, Unit Clin Allergy Res, Dept Med, Stockholm, Sweden
Gripenbäck, S
;
Tornling, G
论文数: 0引用数: 0
h-index: 0
机构:Karolinska Hosp & Inst, Unit Clin Allergy Res, Dept Med, Stockholm, Sweden
Tornling, G
;
Eklund, A
论文数: 0引用数: 0
h-index: 0
机构:Karolinska Hosp & Inst, Unit Clin Allergy Res, Dept Med, Stockholm, Sweden
Eklund, A
;
Scheynius, A
论文数: 0引用数: 0
h-index: 0
机构:Karolinska Hosp & Inst, Unit Clin Allergy Res, Dept Med, Stockholm, Sweden
Scheynius, A
;
Gabrielsson, S
论文数: 0引用数: 0
h-index: 0
机构:Karolinska Hosp & Inst, Unit Clin Allergy Res, Dept Med, Stockholm, Sweden