Scyphostatin, a neutral sphingomyelinase inhibitor from a discomycete, Trichopeziza mollissima:: Taxonomy of the producing organism, fermentation, isolation, and physico-chemical properties

被引:108
作者
Nara, F
Tanaka, M
Hosoya, T
Suzuki-Konagai, K
Ogita, T
机构
[1] Sankyo Co Ltd, Biomed Res Labs, Shinagawa Ku, Tokyo 1408710, Japan
[2] Sankyo Co Ltd, Biol Res Labs, Shinagawa Ku, Tokyo 1408710, Japan
[3] Sankyo Co Ltd, Tsukuba Res Labs, Shinagawa Ku, Tokyo 1408710, Japan
关键词
D O I
10.7164/antibiotics.52.525
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We performed experiments to screen for neutral sphingomyelinase inhibitors using rat brain microsomes as an enzyme source. Among more than 10,000 microbial extracts tested, a mycelial extract of Trichopeziza mollissima SANK 13892 exhibited potent inhibitory activity. The active compound, scyphostatin, was purified by a series of chromatographies. Scyphostatin inhibited the enzyme with an IC50 value of 1.0 mu M.
引用
收藏
页码:525 / 530
页数:6
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