An improved method for the measurement of lidocaine and its metabolites in rat plasma

被引：14

作者：

Rossi, SS ^{[1
]}

Moore, AC ^{[1
]}

Hagey, LR ^{[1
]}

Yaksh, TL ^{[1
]}

Chaplan, SR ^{[1
]}

机构：

[1] UNIV CALIF SAN DIEGO,DEPT MED,LA JOLLA,CA 92093

来源：

THERAPEUTIC DRUG MONITORING | 1997年 / 19卷 / 02期

关键词：

lidocaine; plasma; MEG-X; 3-hydroxylation; glucuronidation; solid-phase extraction; gas chromatography;

D O I：

10.1097/00007691-199704000-00011

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

An improved method is described for the quantitation of lidocaine and its dominant metabolites in rat plasma, 3-hydroxy-lidocaine glucuronide and 3-hydroxy-MEG-X glucuronide. Frozen plasma samples (100-200 mu l) were thawed and deproteinated by precipitation with acetonitrile, before the conversion of glucuronidates into their respective hydroxylated forms by acid hydrolysis. After extraction with solid-phase C-18 cartridge chromatography, the metabolites phorus detection, without derivativization. A detection limit of 0.005 mu g/ml for lidocaine and nonglucuronidated metabolites and 0.01 mu g/ml for glucuronidated metabolites was achieved. The method offers significant improvements in sensitivity relative to existing techniques, which should be of specific benefit to studies in which sample volume is limited, such as those concerned with the pharmacokinetics of lidocaine metabolism in small-animal pain state models.

引用

页码：179 / 184

页数：6

共 12 条

[1] PROLONGED ALLEVIATION OF TACTILE ALLODYNIA BY INTRAVENOUS LIDOCAINE IN NEUROPATHIC RATS [J].