Chemical cleavage sequencing of DNA using matrix-assisted laser desorption ionization time-of-flight mass spectrometry

被引：18

作者：

Isola, NR ^{[1
]}

Allman, SL ^{[1
]}

Golovlov, VV ^{[1
]}

Chen, CH ^{[1
]}

机构：

[1] Oak Ridge Natl Lab, Div Life Sci, Photophys Grp, Oak Ridge, TN 37831 USA

来源：

ANALYTICAL CHEMISTRY | 1999年 / 71卷 / 13期

关键词：

D O I：

10.1021/ac990151o

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper, we report for the first time use of laser desorption mass spectrometry for measurement of chemical cleavage sequencing products of DNA. Tn this method, the target DNA was labeled with biotin and subjected to chemical modification and cleavage according to the Maxam-Gilbert sequencing protocol. The biotin-containing fragments were captured by streptavidin-coated magnetic beads and separated from the other fragments. The captured fragments were released by hot ammonia treatment, and the released fragments were analyzed by mass spectrometry. Potential applications of this method in resolving sequence ambiguities and sequencing repeat sequences as well as in the analysis of DNA-protein interactions are discussed.

引用

页码：2266 / 2269

页数：4

共 27 条

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