Activation of Neutrophils by the Two-Component Leukotoxin LukE/D from Staphylococcus aureus: Proteomic Analysis of the Secretions

被引：4

作者：

Aslam, Rizwan ^{[1
]}

Laventie, Benoit-Joseph ^{[2
]}

Marban, Celine ^{[1
]}

Prevost, Gilles ^{[2
]}

Keller, Daniel ^{[2
]}

Strub, Jean-Marc ^{[3
]}

van Dorsselaer, Alain ^{[3
]}

Haikel, Youssef ^{[1
,4
]}

Taddei, Corinne ^{[4
]}

Metz-Boutigue, Marie-Helene ^{[1
]}

机构：

[1] Univ Strasbourg, INSERM, UMR 1121, F-67000 Strasbourg, France

[2] Univ Strasbourg, CHRU Strasbourg, EA Virulence Bacterienne Precoce Federat Med Tran, F-67000 Strasbourg, France

[3] Univ Strasbourg, CNRS, UMR 7178, F-67000 Strasbourg, France

[4] Univ Strasbourg, Fac Odontol, F-67000 Strasbourg, France

来源：

JOURNAL OF PROTEOME RESEARCH | 2013年 / 12卷 / 08期

关键词：

Staphylococcus aureus; Leukotoxin LukE/D; Neutrophils; Chromogranin A/B; Proteomic Analysis; Antimicrobial Peptides; Innate Immunity; CHROMOGRANIN-A; PROTEIN; GRANULES; PURIFICATION; LEUKOCIDIN; TOXIN; LUKD; ANTIBACTERIAL; MEMBRANE; PEPTIDE;

D O I：

10.1021/pr400199x

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Staphylococcus aureus is responsible for severe bacterial infections in hospitals and healthcare facilities. It produces single and bicomponent toxins (leukotoxins and hemolysins) that hinder innate immune function. Leukotoxin subunits bind to leukocyte cell membrane thus inducing transmembrane pores and subsequently, cell lysis. Leukotoxin LukE/D is a member of the bicomponent toxin family, but to date, no study concerning its involvement in host-pathogen interactions has been reported. In the present study, we performed the proteomic analysis of the secretions recovered after activation of human neutrophils by leukotoxin LukE/D. The neutrophil secretions were purified by RP-HPLC and different fractions were analyzed by Edman sequencing, LC-MS/MS, immunoblotted for chromogranin-derived peptides and further analyzed for antimicrobial properties. Proteomic analysis revealed that neutrophil secretions constitute a large number of proteins related with immune boosting mechanisms, proteolytic degradation, inflammatory process 2% and antioxidant reactions.

引用

页码：3667 / 3678

页数：12

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