Protection against human papillomavirus type 16-induced tumors in mice using non-genetically modified lactic acid bacteria displaying E7 antigen at its surface

被引：48

作者：

Ribelles, Pedro ^{[1
,2
,4
]}

Benbouziane, Bouasria ^{[1
,2
,5
]}

Langella, Philippe ^{[1
,2
]}

Suarez, Juan E. ^{[3
,4
]}

Bermudez-Humaran, Luis G. ^{[1
,2
]}

Riazi, Ali ^{[5
]}

机构：

[1] INRA, Commensal & Probiot Host Interact Lab, UMR Micalis 1319, F-78350 Jouy En Josas, France

[2] AgroParisTech, UMR Micalis, F-78350 Jouy En Josas, France

[3] Inst Prod Lacteos Asturias CSIC, Villaviciosa, Spain

[4] Univ Oviedo, Area Microbiol, Inst Univ Biotecnol, Oviedo, Spain

[5] Univ Mostaganem, Dept Biotechnol, Lab Beneficial Microorganisms Funct Foods & Hlth, Mostaganem 27000, Algeria

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2013年 / 97卷 / 03期

关键词：

Mucosal vaccines; Lactococcus lactis; Lactobacillus casei; Lactic acid bacteria; A2 phage lysin; Cell wall anchor; E7; HPV-16; CONTROLLED GENE-EXPRESSION; LACTOCOCCUS-LACTIS; ESTABLISHED TUMORS; VACCINATION; PEPTIDOGLYCAN; MECHANISMS; REGRESSION; ENDOLYSIN; PROTEINS; DOMAINS;

D O I：

10.1007/s00253-012-4575-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Human papillomavirus (HPV) is the causative agent of cervical cancer (CxCa) and the most commonly sexually transmitted pathogen worldwide. HPV type 16 (HPV-16) E7 oncoprotein is constitutively produced in CxCa and considered as a good antigen candidate for the development of new therapeutic CxCa vaccines. Here, we report the use of non-genetically modified, E7-expressing lactic acid bacteria (LAB) by using the cell-binding domain from Lactobacillus casei A2 phage lysin as a cell wall anchor. The versatility of this system was validated by investigating E7 stability at the surface of Lactococcus lactis and L. casei, two major species of LAB. Moreover, we demonstrated the successful use of these LAB displaying E7 antigen as a mucosal live vaccine in mice. Altogether, these results show the feasibility of using non-genetically modified LAB for low-cost mucosal immunotherapy against HPV-related CxCa in humans.

引用

页码：1231 / 1239

页数：9

共 30 条

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