In vivo pathogenicity and resistance to phagocytosis of Streptococcus equi strains with different levels of capsule expression

被引:40
作者
Anzai, T
Timoney, JF
Kuwamoto, Y
Fujita, Y
Wada, R
Inoue, T
机构
[1] Japan Racing Assoc, Equine Res Inst, Epizoot Res Stn, Kokubunji, Tochigi 3290412, Japan
[2] Univ Kentucky, Dept Vet Sci, Gluck Equine Res Ctr, Lexington, KY 40546 USA
[3] Yamaguchi Univ, Fac Agr, Dept Vet Hyg, Yamaguchi 7530841, Japan
关键词
Streptococcus equi; horse; capsule; phagocytosis; strangles;
D O I
10.1016/S0378-1135(99)00051-6
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The glossy non-encapsulated strain of Steptococcus equi, NCTC 9682, was compared with the matt strain Hidaka/95/2 which expresses a medium sized capsule and with the mucoid CF32 which expresses a large sized capsule in phagocytosis assays and for virulence in inoculated horses. The three strains, NCTC 9682, Hidaka /95/2 and CF32 produced 2.0, 3.1, and 5.3 mg/g wet cells respectively after 3 h incubation, but similar amounts of M-like proteins, cytotoxin and mitogen. NCTC 9682 showed no resistance to phagocytosis by equine neutrophils regardless of the presence of opsonin while strains Hidaka /95/2 and CF32 showed almost complete resistance to phagocytosis. Furthermore, NCTC 9682 produced no clinical disease although it infected the guttural pouch and caused seroconversion. Typical strangles with guttural pouch invasion was observed in all horses infected with encapsulated strains. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:277 / 286
页数:10
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