Interactions among Glomus irregulare, arbuscular mycorrhizal spore-associated bacteria, and plant pathogens under in vitro conditions

被引:58
作者
Bharadwaj, Dharam Parkash [2 ]
Alstrom, Sadhna [2 ]
Lundquist, Per-Olof [1 ]
机构
[1] Swedish Univ Agr Sci, Uppsala BioCtr, Dept Plant Biol & Forest Genet, SE-75007 Uppsala, Sweden
[2] Swedish Univ Agr Sci, Uppsala BioCtr, Dept Mycol & Plant Pathol, SE-75007 Uppsala, Sweden
关键词
Arbuscular mycorrhizal fungi; Exudate analysis; Glomus irregulare; Interactions; Potato pathogens; Spore-associated bacteria; PHYTOPHTHORA-FRAGARIAE; HELPER BACTERIA; HYPHAL GROWTH; FUNGUS; ROOTS; INTRARADICES; RHIZOSPHERE; COLONIZATION; STIMULATION; STRAWBERRY;
D O I
10.1007/s00572-011-0418-7
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Arbuscular mycorrhizal (AM) fungi interact with bacteria (AM fungi-associated bacteria, AMB) in the mycorrhizosphere. We previously identified a set of AMB that enhance AM fungal colonization, plant growth, and inhibit pathogens. Here, we used transformed carrot root cultures in a two-compartment plate system for further in vitro studies on interactions taking place among Glomus irregulare (syn.Glomus intraradices), AMB, and plant pathogens. We found that exudates of G. irregulare stimulated growth of all ten AMB isolates tested in multi-well plates. AMB growth stimulation was observed also during co-cultivation of three of these AMB with G. irregulare in the hyphal compartment. In addition, co-cultivation stimulated growth of G. irregulare hyphae and spore production, as well as G. irregulare root colonization. GC/MS analysis in a preliminary screening of metabolites revealed differences in concentrations of several identified but also unidentified compounds in G. irregulare hyphal exudates. Exudates in presence of three different AMB isolates co-cultivated with G. irregulare contained several additional compounds that differed in amount compared with G. irregulare alone. The results indicate that G. irregulare exudates contain carbohydrates, amino acids, and unidentified compounds that could serve as a substrate to stimulate AMB growth. With regard to effects on plant pathogens, growth inhibition of Rhizoctonia solani, Verticillium dahliae, and Pectobacterium carotovorum ssp. carotovorum was evident in the presence of the AMB isolates tested together with the G. irregulare exudates. These in vitro studies suggest that G. irregulare and AMB stimulate growth of each other and that they together seem to provide an additive effect against growth of both fungal and bacterial pathogens.
引用
收藏
页码:437 / 447
页数:11
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