Calcium oscillations in neocortical astrocytes under epileptiform conditions

被引:73
作者
Tashiro, A [1 ]
Goldberg, J [1 ]
Yuste, R [1 ]
机构
[1] Columbia Univ, Dept Biol Sci, New York, NY 10027 USA
来源
JOURNAL OF NEUROBIOLOGY | 2002年 / 50卷 / 01期
关键词
glia; epilepsy; fura-2;
D O I
10.1002/neu.10019
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Morphological and functional alterations in astrocytic glia are often found in epileptic syndromes, although the exact role of astrocytes in epilepsy is poorly understood. During calcium imaging of epileptiform events in juvenile neocortical slices we previously discovered cells with spontaneous oscillations in their intracellular free calcium concentration ([Ca2+](i)). We have now characterized these oscillations using two in vitro models of epilepsy and find that they are produced by astrocytes. Astrocytic oscillations are widespread throughout the imaged territories, are remarkably regular and have long periods, averaging 100 s, which become shorter during development. Astrocytic oscillations are uncorrelated among themselves and with epileptiform events, are blocked by internal release antagonists and are stimulated by caffeine. Astrocytic calcium oscillations could mediate reactive astrogliosis, contribute to the pathogenesis of chronic epileptic syndromes, and be used as a diagnostic test for epileptic tissue. (C) 2002 John Wiley & Sons, Inc.
引用
收藏
页码:45 / 55
页数:11
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