High levels of foreign gene expression in hepatocytes after tail vein injections of naked plasmid DNA

被引:782
作者
Zhang, GF
Budker, V
Wolff, JA
机构
[1] Univ Wisconsin, Waisman Ctr, Dept Pediat, Madison, WI 53705 USA
[2] Univ Wisconsin, Waisman Ctr, Dept Med Genet, Madison, WI 53705 USA
关键词
D O I
10.1089/10430349950017734
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We have previously shown that the intramuscular injection of naked plasmid DNA enables foreign gene expression in muscle. Further studies showed that the intravascular delivery of naked plasmid DNA enables high levels of expression not only in muscle but also in hepatocytes. For the liver, this technique required injection directly into the liver vessels (portal vein, hepatic vein, or bile duct) and occlusion of outflow. The present study now demonstrates that high levels of plasmid DNA expression in hepatocytes can be easily obtained by tail vein injections. The highest levels of expression are achieved by rapidly injecting the plasmid DNA in large volumes, similar to 2.5 ml. This technique has great potential for a wide variety of laboratory studies.
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收藏
页码:1735 / 1737
页数:3
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