Fine mapping of the Ph-3 gene conferring resistance to late blight (Phytophthora infestans) in tomato

被引：58

作者：

Zhang, Chunzhi ^{[1
]}

Liu, Lei ^{[1
]}

Zheng, Zheng ^{[1
]}

Sun, Yuyan ^{[1
]}

Zhou, Longxi ^{[1
]}

Yang, Yuhong ^{[1
]}

Cheng, Feng ^{[1
]}

Zhang, Zhonghua ^{[1
]}

Wang, Xiaowu ^{[1
]}

Huang, Sanwen ^{[1
]}

Xie, Bingyan ^{[1
]}

Du, Yongchen ^{[1
]}

Bai, Yuling ^{[1
,2
]}

Li, Junming ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China

[2] Univ Wageningen & Res Ctr, Wageningen UR Plant Breeding, NL-6708 PB Wageningen, Netherlands

来源：

THEORETICAL AND APPLIED GENETICS | 2013年 / 126卷 / 10期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

DISEASE RESISTANCE; SOLANUM-VENTURII; POTATO; CLONING; VIRUS; ACCESSION; GENOMICS; HOMOLOG; TM-2(2); CLUSTER;

D O I：

10.1007/s00122-013-2162-1

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Late blight, caused by the oomycete pathogen Phytophthora infestans (Mont.) de Bary, is a devastating disease for tomato and potato crops. In the past decades, many late blight resistance (R) genes have been characterized in potato. In contrast, less work has been conducted on tomato. The Ph-3 gene from Solanum pimpinellifolium was introgressed into cultivated tomatoes and conferred broad-spectrum resistance to P. infestans. It was previously assigned to the long arm of chromosome 9. In this study, a high-resolution genetic map covering the Ph-3 locus was constructed using an F-2 population of a cross between Solanum lycopersicum CLN2037B (containing Ph-3) and S. lycopersicum LA4084. Ph-3 was mapped in a 0.5 cM interval between two markers, Indel_3 and P55. Eight putative genes were found in the corresponding 74 kb region of the tomato Heinz1706 reference genome. Four of these genes are resistance gene analogs (RGAs) with a typical nucleotide-binding adaptor shared by APAF-1, R proteins, and CED-4 domain. Each RGA showed high homology to the late blight R gene Rpi-vnt1.1 from Solanum venturii. Transient gene silencing indicated that a member of this RGA family is required for Ph-3-mediated resistance to late blight in tomato. Furthermore, this RGA family was also found in the potato genome, but the number of the RGAs was higher than in tomato.

引用

页码：2643 / 2653

页数：11

共 58 条

[1]

Agrios G.N., 1997, Plant Pathology, VFourth

[2]

AVDRC, 1999, AVRDC REP 1998, P9

[3]

AVDRC, 1998, AVRDC REP 1997, P83

[4]

AVDRC, 1995, 1994 PROGR REP, P194

[5]

Black L. L., 1996, Phytopathology, V86, pS24

[6]

Black L. L., 1996, TVIS Newsletter, V1, P15

[7]

BONDE REINER, 1952, MAINE AGRIC EXPT STA BULL, V497, P5

[8] QTL analysis of quantitative resistance to Phytophthora infestans (late blight) in tomato and comparisons with potato [J].

Brouwer, DJ ;

Jones, ES ;

St Clair, DA .

GENOME, 2004, 47 (03) :475-492

[9] Fine mapping of three quantitative trait loci for late blight resistance in tomato using near isogenic lines (NILs) and sub-NILs [J].

Brouwer, DJ ;

St Clair, DA .

THEORETICAL AND APPLIED GENETICS, 2004, 108 (04) :628-638

[10] Phenotypic and Genotypic Changes in the Phytophthora infestans Population in Taiwan-1991 to 2006 [J].

Chen, Chien-Hua ;

Wang, Tien-Chen ;

Black, Lowell ;

Sheu, Zong-Ming ;

Perez, Frances ;

Deahl, Kenneth .

JOURNAL OF PHYTOPATHOLOGY, 2009, 157 (04) :248-255

← 1 2 3 4 5 6 →