Selection of a peptide with affinity for the tumor-associated TAG72 antigen from a phage-displayed library

被引:11
作者
Gui, J
Moyana, T
Xiang, J
机构
[1] UNIV SASKATCHEWAN,SASKATOON CANC CTR,DEPT MICROBIOL,SASKATOON,SK S7N 0W0,CANADA
[2] UNIV SASKATCHEWAN,SASKATOON CANC CTR,DEPT PATHOL,SASKATOON,SK S7N 0W0,CANADA
基金
英国医学研究理事会;
关键词
D O I
10.1006/bbrc.1996.0072
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A hexapeptide phage library was used to select peptides with affinity for the tumor-associated TAG72 antigen. Twenty-one phage clones were selected after the third round of biopanning. Three phage clones with the same DNA insert of ARTLRF were found to bind more strongly to the TAG72 antigen than other phage clones and the wild-type phage. A synthetic decapeptide GAARTLRFGA with two conjunctive amino acid residues of the phage coat protein III on each side of the selected peptide was found to bind more strongly to the TAG72 antigen than to other antigens such as the mouse metallothionein. Furthermore, immunohistochemical studies revealed that this peptide displayed preferential binding to colonic adenocarcinomatous cells expressing the TAG72 antigen. Therefore, this anti-TAG72 peptide may be useful in serving as the starting point with regard to further designing peptidomimetics as potential pharmaceuticals. (C) 1996 Academic Press, Inc.
引用
收藏
页码:414 / 419
页数:6
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