Purification and characterization of a novel naphthalene dioxygenase from Rhodococcus sp strain NCIMB12038

被引：89

作者：

Larkin, MJ

Allen, CCR

Kulakov, LA

Lipscomb, DA

机构：

[1] Queens Univ Belfast, Questor Ctr, Belfast BT9 5AG, Antrim, North Ireland

[2] Queens Univ Belfast, Ctr Med Biol, Sch Biol & Biochem, Belfast BT9 7BL, Antrim, North Ireland

来源：

JOURNAL OF BACTERIOLOGY | 1999年 / 181卷 / 19期

关键词：

D O I：

10.1128/JB.181.19.6200-6204.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report here the characterization of the catalytic component (ISPNAR) of a new naphthalene dioxygenase from Rhodococcus sp. strain NCIMB12038. The genes encoding the two subunits of ISPNAR are not homologous to their previously characterized counterparts in Pseudomonas. The deduced amino acid sequences have only 33 and 29% identity with the corresponding subunits in Pseudomonas putida NCIB 9816-4, for which the tertiary structure has been reported.

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页码：6200 / 6204

页数：5

相关论文

共 25 条

[1] Metabolism of naphthalene, 1-naphthol, indene, and indole by Rhodococcus sp strain NCIMB 12038 [J].

Allen, CCR ;

Boyd, DR ;

Larkin, MJ ;

Reid, KA ;

Sharma, ND ;

Wilson, K .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (01) :151-155

[2]

Butler CS, 1997, ADV MICROB PHYSIOL, V38, P47

[3]

Cerniglia Carl E., 1992, Biodegradation, V3, P351, DOI 10.1007/BF00129093

[4] OXIDATION OF NAPHTHALENE BY A MULTICOMPONENT ENZYME-SYSTEM FROM PSEUDOMONAS SP STRAIN NCIB9816 [J].

ENSLEY, BD ;

GIBSON, DT ;

LABORDE, AL .

JOURNAL OF BACTERIOLOGY, 1982, 149 (03) :948-954

[5] NAPHTHALENE DIOXYGENASE - PURIFICATION AND PROPERTIES OF A TERMINAL OXYGENASE COMPONENT [J].

ENSLEY, BD ;

GIBSON, DT .

JOURNAL OF BACTERIOLOGY, 1983, 155 (02) :505-511

[6]

FINNERTY WR, 1992, ANNU REV MICROBIOL, V46, P193, DOI 10.1146/annurev.micro.46.1.193

[7] NAPHTHALENE DEGRADATION VIA SALICYLATE AND GENTISATE BY RHODOCOCCUS SP STRAIN B4 [J].

GRUND, E ;

DENECKE, B ;

EICHENLAUB, R .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (06) :1874-1877

[8] PURIFICATION AND PROPERTIES OF NADH-FERREDOXINNAP REDUCTASE, A COMPONENT OF NAPHTHALENE DIOXYGENASE FROM PSEUDOMONAS SP STRAIN NCIB-9816 [J].

HAIGLER, BE ;

GIBSON, DT .

JOURNAL OF BACTERIOLOGY, 1990, 172 (01) :457-464

[9] FUNCTIONAL AND EVOLUTIONARY RELATIONSHIPS AMONG DIVERSE OXYGENASES [J].

HARAYAMA, S ;

KOK, M ;

NEIDLE, EL .

ANNUAL REVIEW OF MICROBIOLOGY, 1992, 46 :565-601

[10] SULREDOXIN - A NOVEL IRON-SULFUR PROTEIN OF THE THERMOACIDOPHILIC ARCHAEON SULFOLOBUS SP STRAIN-7 WITH A RIESKE-TYPE [2FE-2S] CENTER [J].

IWASAKI, T ;

ISOGAI, Y ;

IIZUKA, T ;

OSHIMA, T .

JOURNAL OF BACTERIOLOGY, 1995, 177 (09) :2576-2582