Microbial signatures can help distinguish moon sponges (family Tetillidae) from Darwin Harbour, Australia

被引:6
作者
Chambers, Kylie [1 ]
Padovan, Anna [1 ]
Alvarez, Belinda [1 ,2 ]
Gibb, Karen [1 ]
机构
[1] Charles Darwin Univ, Res Inst Environm & Livelihoods, Darwin, NT 0909, Australia
[2] Museum & Art Gallery Northern Terr, Darwin, NT 0801, Australia
关键词
COI; DGGE; DNA barcoding; taxonomy; 16S RIBOSOMAL-RNA; COMMUNITY; POPULATIONS; DIVERSITY; PORIFERA;
D O I
10.1071/MF12226
中图分类号
S9 [水产、渔业];
学科分类号
090805 [渔业资源学];
摘要
The bacterial communities of two sponge morphs collected as part of an ecological study and initially allocated to the genus Paratetilla (Demospongiae: Spirophorida: Tetillidae) were analysed using denaturing gradient gel electrophoresis (DGGE) targeting a region of the 16S rRNA gene. The results showed that the two morphs had different bacterial communities, which suggested that they might be distinct Paratetilla species. The sponge samples were further analysed using conventional taxonomy and cytochrome oxidase I (COI) gene sequencing. These data confirmed that (1) the two morphs belonged to different species, and (2) one morph was more closely related to the tetillid genus Cinachyrella than to Paratetilla.
引用
收藏
页码:716 / 725
页数:10
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