Lipoprotein and apolipoprotein secretion by a newborn piglet intestinal cell line (IPEC-1)

被引:73
作者
GonzalezVallina, R
Wang, H
Zhan, RG
Berschneider, HM
Lee, RM
Davidson, NO
Black, DD
机构
[1] UNIV ARKANSAS MED SCI HOSP, ARKANSAS CHILDRENS HOSP, DEPT PEDIAT, LITTLE ROCK, AR 72202 USA
[2] UNIV CHICAGO, PRITZKER SCH MED, DEPT PEDIAT, CHICAGO, IL 60637 USA
[3] UNIV CHICAGO, PRITZKER SCH MED, DEPT MED, CHICAGO, IL 60637 USA
[4] N CAROLINA STATE UNIV, COLL VET MED, RALEIGH, NC 27606 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 1996年 / 271卷 / 02期
关键词
fatty acid; immunoprecipitation; messenger ribonucleic acid editing; radiolabeling; triglyceride;
D O I
10.1152/ajpgi.1996.271.2.G249
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
The aim of these studies was to characterize the synthesis and secretion of lipoproteins and apolipoprotein B (ape B) and apo A-I by a newborn swine intestinal epithelial cell line (IPEC-1). Differentiated cells exhibited enterocytic features, including microvilli. [H-3]oleic acid was taken up and incorporated into cellular lipids and secreted into the basolateral medium in lipoproteins. Total apo B and apo A-I secreted increased with oleic acid incubation. However, cellular apo B and apo A-I content did not change. Whereas undifferentiated cells synthesized and secreted only apo B-100, both apo B-100 and apo B-48 were produced by differentiated cells. The ratio of radiolabeled apo B-48 to apo B-100 in both basolateral medium and cell homogenate increased with oleic acid treatment after 24-h steady-state labeling. However, apo B mRNA editing was unchanged, indicating posttranslational regulation of this ratio. Pulse-chase radiolabeling demonstrated no major changes in cellular or basolateral medium apolipoprotein labeling kinetics with oleic acid or dexamethasone incubation. The dissociation of apo B and apo A-I mass secretion from the secretion of radiolabeled apo B and apo A-I in response to oleic acid absorption suggests the presence of an intracellular pool of apolipoprotein with a slow turnover that is mobilized for secretion in response to fatty acid uptake.
引用
收藏
页码:G249 / G259
页数:11
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