Manipulating gene expression for the metabolic engineering of plants

被引:67
作者
Lessard, PA
Kulaveerasingam, H
York, GM
Strong, A
Sinskey, AJ
机构
[1] MIT, Dept Biol, Cambridge, MA 02139 USA
[2] Univ Putra Malaysia, Genome Ctr, Inst Biosci, Serdang, Selangor Darul, Malaysia
关键词
D O I
10.1006/mben.2001.0210
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Introducing and expressing foreign genes in plants present many technical challenges that are not encountered with microbial systems. This review addresses the variety of issues that must be considered and the variety of options that are available, in terms of choosing transformation systems and designing recombinant transgenes to ensure appropriate expression in plant cells. Tissue specificity and proper developmental regulation, as well as proper subcellular localization of products, must be dealt with for successful metabolic engineering in plants. (C) 2002 Elsevier Science.
引用
收藏
页码:67 / 79
页数:13
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