High-performance liquid chromatography of HIV protease inhibitors in human biological matrices

Methods for HPLC analysis of protease inhibitors (Pls) in human biological matrices were reviewed. Assays have been developed for analysis of single PIs or for simultaneous measurement of multiple PIs in plasma-serum, saliva, cerebrospinal fluid and semen. Liquid-liquid extraction was most often applied for sample pretreatment but solid-phase extraction and protein precipitation were used as well. Reversed-phase or ion-pair chromatography have been used to separate PIs. Detection of Pls should be sensitive enough for quantitation of plasma concentrations below trough levels of single Pls, or below proposed therapeutic thresholds for Pls. The large majority of assays employs UV detection. As the potential for interferences is large, the selectivity of every method should be evaluated properly. The available high-performance liquid chromatography (HPLC) methods have been applied in clinical pharmacokinetic studies and for therapeutic drag monitoring of Pls. Participation in an interlaboratory quality control program is recommended for every laboratory engaged in the bioanalysis of PIs. (C) 2001 Elsevier Science B.V. All rights reserved.